hsa-miR-145-5p Two-Tailed PRIMERs

Cat. No.	Size	Price
RDTT0000437PRI	50 rxn/ 150 rxn

Technical Data

Don't forget

For successful completion of the assay, we strongly recommend purchasing of our Two-Tailed cDNA Synthesis System and Two-Tailed qPCR Master Mix. The combination of these three products will provide you with the highest quality results.

Type

Two-Tailed RT/ PCR Primers

Shipping

Frozen. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).

Note

Below, links to independent papers developing and using Two-Tailed PCR can be found

Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
Click here for more papers citing Two-tailed PCR

Summary

Features

For research use only.
For measurements specific miRNA in biological fluids or tisues.
Components of the kit are provided ready to use.

Research topic

Cardiovascular disease, Oncology

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

miR-145-5p is considered a member of the p53-tumor suppressor network and has been found to act as a post-transcriptional regulator of many cancer-related genes. Deregulations of miR-145-5p were observed in various types of human cancer including renal cancer, colorectal cancer, bladder cancer, lung cancer, glioblastoma and prostate cancer.

miR-145-5p is also involved in pathology of cardiovascular disease. miR-145-5p plasma levels were reported to be significantly lower in patients with acute myocardial infarction compared to healthy control group. Increased plasma levels of miR-145-5p were found in population with genetic predisposition to familiar dilated cardiomyopathy.

miR-145-5p is associated also with cerebral ischemia, where expression was significally higher in ischemic stroke patients when compare to control subjects.

Summary References (9)

References to miR-145-5p

Papadopoulos, Emmanuel I., et al. "Clinical evaluation of microRNA-145 expression in renal cell carcinoma: a promising molecular marker for discriminating and staging the clear cell histological subtype." Biological chemistry 397.6 (2016): 529-539.
Li, Jialu, et al. "Serum miRNA expression profile as a prognostic biomarker of stage II/III colorectal adenocarcinoma." Scientific reports 5 (2015): 12921.
Inamoto, Teruo, et al. "A Panel of MicroRNA Signature as a Tool for Predicting Survival of Patients with Urothelial Carcinoma of the Bladder." Disease Markers 2018 (2018).
Zhang, Hui, et al. "Plasma miR-145, miR-20a, miR-21 and miR-223 as novel biomarkers for screening early-stage non-small cell lung cancer." Oncology letters 13.2 (2017): 669-676.
Zhao, Hua, et al. "Serum microRNA profiling in patients with glioblastoma: a survival analysis." Molecular cancer 16.1 (2017): 59.
Goto, Yusuke, et al. "Impact of novel miR-145-3p regulatory networks on survival in patients with castration-resistant prostate cancer." British journal of cancer 117.3 (2017): 409.
Zhang, Ming, et al. "Circulating MicroRNA-145 is associated with acute myocardial infarction and heart failure." Chinese medical journal 130.1 (2017): 51.
Toro, Rocío, et al. "Plasma microRNAs as biomarkers for Lamin A/C-related dilated cardiomyopathy." Journal of Molecular Medicine 96.8 (2018): 845-856
Gan, C. S., C. W. Wang, and K. S. Tan. "Circulatory microRNA-145 expression is increased in cerebral ischemia." Genet Mol Res 11.1 (2012): 147-152.

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