Type
Two-Tailed RT/ PCR Primers
Shipping
Frozen. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).
Note
Below, links to independent papers developing and using Two-Tailed PCR can be found
- Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
- Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
- Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
- Click here for more papers citing Two-tailed PCR
Features
- For research use only.
- For measurements specific miRNA in biological fluids or tissues.
- Components of the kit are provided ready to use.
Research topic
Immunology, Oncology, Others
Summary
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in the pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.
hsa-miR-150 is encoded by the gene located on the human chromosome 19q13. hsa-miR-150 is selectively expressed in lymph nodes, spleen, and in mature B- and T-cells, and is an important regulator of differentiation and activation of immune cells. It is also involved in the innate and adaptive immune responses. hsa-miR-150 is one of the most studied miRNA and plays an important role in pathogenesis of a number of tumours.
Low levels of hsa-miR-150 in pacient's tumor samples predicted shorter survival and worse response to adjuvant chemotherapy; in contrast, high expression of hsa-miR-150 in pacient's tumor samples indicates better prognosis and better response to adjuvant chemotherapy.
Moreover, over-expression of hsa-miR-150-5p leads to reduced migration and invasion of colorectal carcinoma cells. hsa-miR-150-5p also acts as a metastasis suppressor in colorectal carcinoma.
Plasma levels of hsa-miR-150 were downregulated in patients with acute myeloid leukemia.
hsa-miR-150-5p was upregulated in tumor tissues of non-small-cell lung carcinoma (NSLC) patients and was negatively correlated with expression of the tumor-suppressor geneTP53.
High levels of hsa-miR-150 were observed in gastric cancer tissue. hsa-miR-150 over-expression also promoted gastric cancer proliferation. Furthermore, over-expression of hsa-miR-150 was found in osteosarcoma tumor samples.
