hsa-miR-16-5p miREIA

Cat. No.	Size	Price
Clearance sale RDM0008H	96 wells (1 kit)

Technical Data

Type

miREIA – miRNA enzyme immunoassay

Applications

Whole blood, PBMC, Cell culture lysates

Sample Requirements

10 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

12.5 – 0.39 amol/μl

Limit of Detection

0.13 amol/μl

Intra-assay (Within-Run)

n = 8,
CV = 4.6%

Inter-assay (Run-to-Run)

n = 5,
CV = 8.6%

Spiking Recovery

104.6%

Dilution Linearity

89.2%

Specificity

Crossreactivity with the miRNA family members exhibiting high sequence identity cannot be excluded.

Note

Product Manual: miREIA - microRNA enzyme immunoassay

Have you bought miREIA kits and need help with assay procedure? Please look at product manual video how easy using the miREIA method is.

Product Manual: miREIA - microRNA enzyme immunoassay

Homology

Exact inter-species homology was found for example for:

Bonobo
Brown anole
Brown bat
Brown woolly monkey
Carolina anole
Chimpanzee
Chinese hamster
Dog
Geoffroy's spider monkey

Horse
Mouse
Platypus
Rat
Salmon
Short-tailed opossum
Western gorilla
White-lipped tamarin
Wild boar

Summary

Features

It is intended for research use only
The total assay time is less than 2.5 hours
The kit measures hsa-miR-16-5p isolated from human blood
Assay format is 96 wells
Standard is synthetic miRNA-based
Components of the kit are provided ready to use, concentrated or dried

Research topic

Autoimmunity, Immune Response, Infection and Inflammation, Oncology

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

miR-16, located on the 13q14 chromosome, was initially found to be related to chronic lymphocytic leukemia. Studies reveal that miR-16 is down-regulated and plays a tumor suppressive role by affecting cell proliferation, cell cycle, invasion and apoptosis in different types of malignancies, such as breast cancer, lung cancer, ovarian cancer, osteosarcoma and prostate cancer. In contrast, other studies have reported that miR-16 is up-regulated in renal cell carcinoma and espohageal squamous cell carcinoma where it acts as an oncogene by inducing cellular proliferation and migration and reducing apoptosis.

The level of miR-16-5p was significantly decreased in plasma of systemic lupus erythematosus patients compared with healthy controls and also in serum of patients with rheumatoid arthritis compared to healthy controls.

Summary References (10)

References to miR-16-5p

Humplikova, Lenka, et al. "Expression of miR-15a and miR-16-1 in patients with chronic lymphocytic leukemia." Biomedical Papers 157.4 (2013): 284-293.
Mobarra, Naser, et al. "Overexpression of microRNA-16 declines cellular growth, proliferation and induces apoptosis in human breast cancer cells." In Vitro Cellular & Developmental Biology-Animal 51.6 (2015): 604-611.
Zhu, Yi, et al. "MiR-16 induced the suppression of cell apoptosis while promote proliferation in esophageal squamous cell carcinoma." Cellular Physiology and Biochemistry 33.5 (2014): 1340-1348.
Fu, Xiafei, et al. "MicroRNA-16 Promotes Ovarian Granulosa Cell Proliferation and Suppresses Apoptosis Through Targeting PDCD4 in Polycystic Ovarian Syndrome." Cellular Physiology and Biochemistry 48.2 (2018): 670-682.
Bonci, Désirée, et al. "The miR-15a–miR-16-1 cluster controls prostate cancer by targeting multiple oncogenic activities." Nature medicine 14.11 (2008): 1271.
Jiao, Z. H., J. D. Wang, and X. J. Wang. "MicroRNA-16 suppressed the invasion and migration of osteosarcoma by directly inhibiting RAB23." European review for medical and pharmacological sciences 22.9 (2018): 2598-2605.
Bandi, Nora, et al. "miR-15a and miR-16 are implicated in cell cycle regulation in a Rb-dependent manner and are frequently deleted or down-regulated in non–small cell lung cancer." Cancer research 69.13 (2009): 5553-5559.
Chen, Duqun, et al. "Upregulated microRNA-16 as an oncogene in renal cell carcinoma." Molecular medicine reports 12.1 (2015): 1399-1404.
Zhang, Huidi, et al. "B cell-related circulating microRNAs with the potential value of biomarkers in the differential diagnosis, and distinguishment between the disease activity and lupus nephritis for SLE." Frontiers in immunology 9 (2018): 1473.
Dunaeva, Marina, et al. "Circulating serum miR‐223‐3p and miR‐16‐5p as possible biomarkers of early rheumatoid arthritis." Clinical & Experimental Immunology (2018).

By Research Topic

Autoimmunity
Immune Response, Infection and Inflammation
Oncology

By Molecule

miR-16-5p

By Technical Data

Docs

Product Datasheet (RUO)

Datasheet PDF (RUO)

Safety Information (RUO)

MSDS (RUO)