Two-Tailed RT/ PCR Primers
Frozen. Upon receipt, store the product at the temperature recommended below.
Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).
Below, links to independent papers developing and using Two-Tailed PCR can be found
- Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
- Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
- Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
- Click here for more papers citing Two-tailed PCR
- For research use only.
- For measurements specific miRNA in biological fluids.
- Components of the kit are provided ready to use.
Cardiovascular disease, Oncology, Neurodegenerative disease
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.
MiR-26b is located in an intron of the Ctdsp2 gene and it can regulate neuronal differentiation together with its host gene. MiR-26b has been reported to be a critical regulator in carcinogenesis and tumor progression by acting as a tumor suppressor gene in various types of cancer. It has been found that miR-26b is downregulated in breast cancer and that it can inhibit cellular proliferation. Down-regulation of miR-26b in osteosarcoma increased the levels of CTGF and Smad1, facilitating osteosarcoma metastasis. MiR-26b could also modulate non-small cell lung cancer chemoresistance and migration through its association with PTEN. In addition, a recent study demonstrated that miR-26b-5p suppresses proliferation, migration and invasion of intrahepatic cholangiocarcinoma cells by suppressing S100A7. Similarly, downregulation of miR-26b-5p together with miR-26a-5p was frequently observed in bladder cancer cells, and both of these miRNAs significantly inhibited cancer cell migration and invasion.
Besides cancer, aberrant expression and functional abnormalities of miR-26b have been reported in a variety of other diseases. The results suggest that miR-26b plays a role in maintenance of heart function by regulating Wnt pathway.
miR-26 has been observed to be upregulated in the human temporal cortex in Alzheimer's disease and in APP/PS1 double-transgenic mice, suggesting that miR-26b may function in development of Alzheimer's disease.