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Manufactured by BioVendor

hsa-miR-31-5p Two-Tailed PRIMERs

  • Regulatory status:RUO
  • Type:Two-Tailed RT/ PCR Primers
  • Species:Human
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Cat. No. Size Price


New RDTT0000089RTPRI 50 rxn/ 150 rxn
PubMed Product Details
Technical Data

Don't forget

For successful completion of the assay, we strongly recommend purchasing of our Two-Tailed cDNA Synthesis System and Two-Tailed qPCR Master Mix. The combination of these three products will provide you with the highest quality results.

Type

Two-Tailed RT/ PCR Primers

Shipping

Frozen. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).

Note

Below, links to independent papers developing and using Two-Tailed PCR can be found

  • Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
  • Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
  • Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
  • Click here for more papers citing Two-tailed PCR
Summary

Features

  • For research use only.
  • For measurements specific miRNA in biological fluids.
  • Components of the kit are provided ready to use.

Research topic

Cardiovascular disease, Oncology

Summary

MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in the pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

miRNA-31, including its mature forms miR-NA-31-3p and miRNA-31-5p, has a dual role, both oncogenic and tumor-suppressing, being disrupted in many human cancers. Aberrant expression of miR-31-5p has been detected in various cancers and plays a significant role in tumorigenesis. Low miR-31-5p expression was present in nasopharyngeal carcinoma tissues and cell lines and acted as a tumor suppressive miRNA and low expression of miR-31-5p was highly correlated with tumor-node-metastasis stage. Serum miR-31-5p levels were significantly different between oral cancer patients and healthy controls and between pre- and postoperative patients. Furthermore, a miR-31-5p mimic enhanced the proliferation of normal epithelial cells, and antagomiR-31-5p inhibited the proliferation of oral cancer cells. miR-31-5p was proven to have oncogenic properties in both colorectal cancer (CRC) cell lines and primary colorectal tumors. In CRC, miR-31-3p and miR-31-5p dysregulation seems to have a particular role in response to treatment with anti-EGFR therapy. miR-31-5p was significantly down-regulated in renal cell carcinoma (RCC) tissues and cell lines compared with paired adjacent normal tissues and normal cell lines. miR-31-5p downregulation was associated with poor prognosis in RCC patients. Overexpression of miR-31-5p inhibited RCC cell proliferation, migration and invasion and cell cycle.

Besides cancer, it was found that miR-31-5p was up-regulated in sera from patients with preeclampsia and in human endothelial cells treated with TNFα. TNFα-mediated induction of miR-31-5p was blocked by an NF-κB inhibitor. It was suggest that NF-κB-responsive miR-31-5p elicits endothelial dysfunction, hypertension, and vascular remodeling via post-transcriptional down-regulation of eNOS and is a molecular risk factor in the pathogenesis and development of preeclampsia.

Summary References (5)

References to miR-31-5p

  • Kim S, Lee KS, Choi S, Kim J, Lee DK, Park M, Park W, Kim TH, Hwang JY, Won MH, Lee H, Ryoo S, Ha KS, Kwon YG, Kim YM. NF-κB-responsive miRNA-31-5p elicits endothelial dysfunction associated with preeclampsia via down-regulation of endothelial nitric-oxide synthase. J Biol Chem. 2018 Dec 7;293(49):18989-19000. doi: 10.1074/jbc.RA118.005197. Epub 2018 Oct 2. PMID: 30279269; PMCID: PMC6295733. See more on PubMed
  • Lei SL, Zhao H, Yao HL, Chen Y, Lei ZD, Liu KJ, Yang Q. Regulatory roles of microRNA-708 and microRNA-31 in proliferation, apoptosis and invasion of colorectal cancer cells. Oncol Lett. 2014 Oct;8(4):1768-1774. doi: 10.3892/ol.2014.2328. Epub 2014 Jul 9. PMID: 25202407; PMCID: PMC4156175. See more on PubMed
  • Li Y, Quan J, Chen F, Pan X, Zhuang C, Xiong T, Zhuang C, Li J, Huang X, Ye J, Zhang F, Zhang Z, Gui Y. MiR-31-5p acts as a tumor suppressor in renal cell carcinoma by targeting cyclin-dependent kinase 1 (CDK1). Biomed Pharmacother. 2019 Mar;111:517-526. doi: 10.1016/j.biopha.2018.12.102. Epub 2018 Dec 28. PMID: 30597305. See more on PubMed
  • Lu Z, He Q, Liang J, Li W, Su Q, Chen Z, Wan Q, Zhou X, Cao L, Sun J, Wu Y, Liu L, Wu X, Hou J, Lian K, Wang A. miR-31-5p Is a Potential Circulating Biomarker and Therapeutic Target for Oral Cancer. Mol Ther Nucleic Acids. 2019 Jun 7;16:471-480. doi: 10.1016/j.omtn.2019.03.012. Epub 2019 Apr 11. PMID: 31051332; PMCID: PMC6495075. See more on PubMed
  • Yi SJ, Liu P, Chen BL, Ou-Yang L, Xiong WM, Su JP. Circulating miR-31-5p may be a potential diagnostic biomarker in nasopharyngeal carcinoma. Neoplasma. 2019 May 11;2019:181109N847. PMID: 31088103. See more on PubMed
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