Type
Recombinant protein
Description
Recombinant human angiotensin-converting enzyme 2 (ACE2) extracellular domain (18-615) was expressed in CHO cells using a C-terminal Fc-tag and binds to the SARS Coronavirus 2 (COVID-19) receptor binding domain (RBD).
Source
CHO
Purity
Purified to greater than 95% purity.
SDS-PAGE Gel
- MW Marker
- ACE2 (18-615)
Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized human ACE2 (18-615) protein at 2µg/ml can bind to SARS-CoV-2 Spike protein RBD.
Formulation
Presented in 50mM Tris-HCl, pH7.5, 90mM glycine.
Reconstitution
Defrost at ambient temperature
Applications
Western blotting, ELISA
Shipping
On ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store freezed protein at min. -70°C. The protein remains stable until the expiry date when stored at -70°C.
Quality Control Test
Bradford protein assay to determine quantity of the protein.
SDS-PAGE to determine purity of the protein.
Note
Products are for Research Use or for Further Manufacturing Use only. Not for Diagnostic or Therapeutic Use.
Research topic
Blood pressure regulation and NO metabolism, Cardiovascular disease, Immune Response, Infection and Inflammation, Pulmonary diseases, Renal disease, COVID-19
Summary
ACE-2 (Angiotensin converting enzyme 2) an enzyme bound to cell membranes in various organs such as intestines arteries , lungs, heart & kidney. ACE2 an entry receptor of SARS coronaviruses as well as SARS-CoV-2,.The coronavirus spike (S) glycoprotein is a class I viral fusion antigen located on the external envelope of the virion that takes part in a critical part in viral infection by identifying host cell receptors and facilitating fusion of the viral and cellular membranes. 2 main domains in coronavirus S1 have been recognized, the N-terminal domain and C-terminal domain. One or the other and/or both S1 domains function as a receptor-binding domain. SARS-CoV + MERS-CoV equally use C-domain to attach their receptors.ACE2 is a type I transmembrane antigen with an extracellular N-terminal domain having the catalytic site and an intracellular C-terminal tail. ACE2 obtains a signal peptide, a transmembrane domain, and a single metalloproteinase active site containing an HEXXH zinc-binding domain. ACE-2 plays a role as a mono-carboxypeptidase which degrades Ang I to produce the nonapeptide Ang 1–9 and Ang II to create the heptapeptide Ang 1–7.
