Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma, Cell culture supernatant
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).
Calibration Range
6.14 - 1 500 pg/ml
Limit of Detection
6 pg/ml
Intra-assay (Within-Run)
CV <10%
Inter-assay (Run-to-Run)
CV <12%
Spiking Recovery
serum 95.71%, plasma 84.91%, cell culture media 92.69%
Dilution Linearity
serum 129.3%, plasma 122.2%, cell culture media 135.1%
Specificity
This ELISA antibody pair detects human CHI3L1. Other species not determined.
Features
- RUO
- calibration range 6.14 - 1 500 pg/ml
- limit of detection 6 pg/ml
- intra-assay CV <10%
- inter-assay CV < 12%
Research topic
Immune Response, Infection and Inflammation
Summary
The BioVendor Human CHI3L1 (Chitinase 3-like 1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CHI3L1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human CHI3L1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CHI3L1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CHI3L1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CHI3L1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Instructions for Use (RUO)
Instructions for Use (RUO)
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