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Manufactured by BioVendor


  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:CHI3L1, YKL-40
  • Species:Human
Cat. No. Size Price

New REH016R 96 wells (1 kit) $518,5
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


Serum, Plasma, Cell culture supernatant


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

6.14 - 1 500 pg/ml

Limit of Detection

6 pg/ml

Intra-assay (Within-Run)

CV <10%

Inter-assay (Run-to-Run)

CV <12%

Spiking Recovery

serum 95.71%, plasma 84.91%, cell culture media 92.69%

Dilution Linearity

serum 129.3%, plasma 122.2%, cell culture media 135.1%


This ELISA antibody pair detects human CHI3L1. Other species not determined.



  • RUO
  • calibration range 6.14 - 1 500 pg/ml
  • limit of detection 6 pg/ml
  • intra-assay CV <10%
  • inter-assay CV < 12%

Research topic

Immune Response, Infection and Inflammation


The BioVendor Human CHI3L1 (Chitinase 3-like 1) ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CHI3L1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human CHI3L1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CHI3L1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CHI3L1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CHI3L1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Summary References (3)

References to Chitinase 3-Like 1

  • Dela Cruz CS, Liu W, He CH, Jacoby A, Gornitzky A, Ma B, Flavell R, Lee CG, Elias JA. Chitinase 3-like-1 promotes Streptococcus pneumoniae killing and augments host tolerance to lung antibacterial responses. Cell Host Microbe. 2012 Jul 19;12 (1):34-46
  • Gong Z, Xing S, Zheng F, Xing Q. Increased expression of chitinase 3-like 1 in aorta of patients with atherosclerosis and suppression of atherosclerosis in apolipoprotein E-knockout mice by chitinase 3-like 1 gene silencing. Mediators Inflamm. 2014;2014:905463
  • Libreros S, Garcia-Areas R, Keating P, Carrio R, Iragavarapu-Charyulu VL. Exploring the role of CHI3L1 in "pre-metastatic" lungs of mammary tumor-bearing mice. Front Physiol. 2013;4:392
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