Cat # changed from RMEE05R to E05
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma-EDTA, Urine, Cerebrospinal fluid, Amniotic fluid, Milk, Saliva
Sample Requirements
15 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
2–80 ng/ml
Limit of Detection
0.2 ng/ml
Note
The kits are CE-IVD certified and intended for professional use.
Features
- Measures total IGFBP-2 concentration in serum, plasma and in other human body fluids
- single standards with 2; 10; 20; 40 and 80 ng/ml are
- Total Incubation time only 1.75 hours
- 2 Control Sera are provided for quality control purposes according GLP
- ready-to-use Antibody Conjugate
- no sample extraction needed
- Microtiterplate seperately break apart
Research topic
Growth hormone and factor-related products, Oncology
Summary
Insulin-like growth factors (IGFs) regulate the proliferation, differentation, apoptose, cell adhesion and metabolism in various tissues and cell types. The IGF-1, which is produced mainly in liver under the influence of growth hormone (GH), regulates as hormone the linear growth of the bones and the process of sexual maturity, while IGF-2 is mainly a growth factor of fetal tissue. The biological actions of IGF over the IGF-Type-1 receptor are modulated variably through the IGF binding proteins (IGFBP-1 to-6). IGFBP-2 is, after IGFBP-3, the second most frequent IGFBP in the human blood. IGFs, especially tumor typical pro-IGF-forms and hormones regulate the expression of IGFBP-2, GH effect is thereby inhibiting. At cellular level IGFBP-2 seems to stimulate the proliferation and dissemination of solid tumors via an IGF-independent mechanism.
IGFBP-2 is a unglycosylated polypetide of 31.3 kDa, which forms binary IGF-complexes and shows no circadian rhythm in the circulation.The serum concentration of IGFBP-2 increases in fasting, after major surgery and after trauma, but the increasing of the concentration is most intensive in malignant diseases. The correlation of the IGFBP-2 level to the degree of progression is a striking feature in various tumor types as is the normalization of the IGFBP-serum levels after remission. The IGFBP-2 concentration is age-dependent in blood.
Find documents for the lot
Example Instructions for Use (RUO)
Example Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
MSDS (RUO)
Product Brochure
CE IVD Assays
Other Documents
Declaration of Conformity
