Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Cell culture supernatant
Sample Requirements
50 µl/well
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
7.8–500 pg/ml
Limit of Detection
1.2 pg/ml
Intra-assay (Within-Run)
CV = 4.7%
Inter-assay (Run-to-Run)
CV = 5.7%
Spiking Recovery
77,00%
Dilutation Linearity
109,00%
Research topic
Cytokines and chemokines and related molecules, Oncology
Summary
Interleukin-1 (IL-1), originally described in 1972 as lymphocyte activating factor (LAF) for its effects on thymocytes, is a polypeptide cytokine with two molecular forms. Both forms appear to mediate identical ranges of biological activity which include synthesis of the acute phase proteins by hepatocytes, chemotaxis of polymorphonucleocytes, and release of polymorphonucleocytes from blood and bone marrow. These effects coined the acronym leukocyte endogenous mediator (LEM). Early researchers also called IL-1ß endogenous pyrogen, and it has been shown to induce fever and is thought to contribute to wasting of muscles (PIF, proteolysis inducing factor). Other activities associated with IL-1 are the induction of Prostaglandin E2 by synovial cells and release of collagenase with resulting destruction of cartilage and bone resorption (catabolin, osteoclast activation factor). In addition, IL-1, has multiple immunological functions including enhancement of IL-2 production by T cells and activation of B-cells (BAF) and thymocytes. A true pleiotrope, IL-1 may have tumoricidal activity via its release of IL-2 and interferon gamma and be indirectly antiviral by stimulating fibroblasts to release interferon beta. In its role as mediator of sepsis, IL-1 has most recently been described as enhancing the growth of virulent E. coli.
