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Interleukin-8 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-8, Monocyte-derived neutrophil chemotactic factor, T-cell chemotactic factor, Neutrophil-activating protein 1, Granulocyte chemotactic protein 1
  • Species:Human
Cat. No. Size Price

RD194558200R 96 wells (1 kit) $474
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Saliva


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1.6 – 100 pg/ml

Limit of Detection

0.5 pg/ml

Intra-assay (Within-Run)

n = 8; CV = 4.5 %

Inter-assay (Run-to-Run)

n = 5; CV = 7.2 %

Spiking Recovery

94.6 %



  • It is intended for research use only
  • The total assay time is less than 4 hours
  • The kit measures total human IL-8 in serum, plasma (EDTA, citrate, heparin) and saliva
  • Assay format is 96 wells
  • Standard is purified E.coli protein
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Cytokines and chemokines and related molecules, Immune Response, Infection and Inflammation, Oncology, Sepsis


IL-8 (8 kDa, 72 amino acids) is a non-glycosylated protein and it is produced by the processing of a precursor protein of 99 amino acids. IL-8 is present in the synovial fluids of patients with rheumatoid arthritis and has been purified from plaque psoriasis scales. It is associated with sepsis, asthma and glomerulonephritis.

Product References (1)


  • Kwiatkowski P, Wojciuk B, Wojciechowska-Koszko I, Łopusiewicz Ł, Grygorcewicz B, Pruss A, Sienkiewicz M, Fijałkowski K, Kowalczyk E, Dołęgowska B. Innate Immune Response against Staphylococcus aureus Preincubated with Subinhibitory Concentration of trans-Anethole. Int J Mol Sci. 2020 Jun 11;21(11):4178. doi: 10.3390/ijms21114178. PubMed PMID: 32545315. PubMed CentralPMCID: PMC7312609. See more on PubMed
Summary References (20)

References to Interleukin-8

  • Baggiolini M, Clark-Lewis I. Interleukin-8, a chemotactic and inflammatory cytokine. FEBS Lett. 1992 Jul 27;307 (1):97-101
  • Brat DJ, Bellail AC, Van Meir EG. The role of interleukin-8 and its receptors in gliomagenesis and tumoral angiogenesis. Neuro Oncol. 2005 Apr;7 (2):122-33
  • Burrows SD, Doyle ML, Murphy KP, Franklin SG, White JR, Brooks I, McNulty DE, Scott MO, Knutson JR, Porter D, et al. Determination of the monomer-dimer equilibrium of interleukin-8 reveals it is a monomer at physiological concentrations. Biochemistry. 1994 Nov 01;33 (43):12741-5
  • DeForge LE, Fantone JC, Kenney JS, Remick DG. Oxygen radical scavengers selectively inhibit interleukin 8 production in human whole blood. J Clin Invest. 1992 Nov;90 (5):2123-9
  • DeForge LE, Preston AM, Takeuchi E, Kenney J, Boxer LA, Remick DG. Regulation of interleukin 8 gene expression by oxidant stress. J Biol Chem. 1993 Dec 05;268 (34):25568-76
  • Donnelly SC, Strieter RM, Kunkel SL, Walz A, Robertson CR, Carter DC, Grant IS, Pollok AJ, Haslett C. Interleukin-8 and development of adult respiratory distress syndrome in at-risk patient groups. Lancet. 1993 Mar 13;341 (8846):643-7
  • Holmes WE, Lee J, Kuang WJ, Rice GC, Wood WI. Structure and functional expression of a human interleukin-8 receptor. Science. 1991 Sep 13;253 (5025):1278-80
  • Huang GT, Potente AP, Kim JW, Chugal N, Zhang X. Increased interleukin-8 expression in inflamed human dental pulps. Oral Surg Oral Med Oral Pathol. 1999 Aug;88 (2):214-20
  • Kauma S, Takacs P, Scordalakes C, Walsh S, Green K, Peng T. Increased endothelial monocyte chemoattractant protein-1 and interleukin-8 in preeclampsia. Obstet Gynecol. 2002 Oct;100 (4):706-14
  • Lemster BH, Carroll PB, Rilo HR, Johnson N, Nikaein A, Thomson AW. IL-8/IL-8 receptor expression in psoriasis and the response to systemic tacrolimus (FK506) therapy. Clin Exp Immunol. 1995 Feb;99 (2):148-54
  • Livaditi O, Kotanidou A, Psarra A, Dimopoulou I, Sotiropoulou C, Augustatou K, Papasteriades C, Armaganidis A, Roussos C, Orfanos SE, Douzinas EE. Neutrophil CD64 expression and serum IL-8: sensitive early markers of severity and outcome in sepsis. Cytokine. 2006 Dec;36 (5-6):283-90
  • Matsushima K, Oppenheim JJ. Interleukin 8 and MCAF: novel inflammatory cytokines inducible by IL 1 and TNF. Cytokine. 1989 Nov;1 (1):2-13
  • Mukaida N. Pathophysiological roles of interleukin-8/CXCL8 in pulmonary diseases. Am J Physiol Lung Cell Mol Phy. 2003 Apr;284 (4):L566-77
  • Mukaida N, Harada A, Yasumoto K, Matsushima K. Properties of pro-inflammatory cell type-specific leukocyte chemotactic cytokines, interleukin 8 (IL-8) and monocyte chemotactic and activating factor (MCAF). Microbiol Immunol. 1992;36 (8):773-89
  • Mukaida N, Morita M, Ishikawa Y, Rice N, Okamoto S, Kasahara T, Matsushima K. Novel mechanism of glucocorticoid-mediated gene repression. Nuclear factor-kappa B is target for glucocorticoid-mediated interleukin 8 gene repression. J Biol Chem. 1994 May 06;269 (18):13289-95
  • Paolini JF, Willard D, Consler T, Luther M, Krangel MS. The chemokines IL-8, monocyte chemoattractant protein-1, and I-309 are monomers at physiologically relevant concentrations. J Immunol. 1994 Sep 15;153 (6):2704-17
  • Sharma A, Satyam A, Sharma JB. Leptin, IL-10 and inflammatory markers (TNF-alpha, IL-6 and IL-8) in pre-eclamptic, normotensive pregnant and healthy non-pregnant women. Am J Reprod Immunol. 2007 Jul;58 (1):21-30
  • Teles RP, Likhari V, Socransky SS, Haffajee AD. Salivary cytokine levels in subjects with chronic periodontitis and in periodontally healthy individuals: a cross-sectional study. J Periodontal Res. 2009 Jun;44 (3):411-7
  • Tsai CC, Ho YP, Chen CC. Levels of interleukin-1 beta and interleukin-8 in gingival crevicular fluids in adult periodontitis. J Periodontol. 1995 Oct;66 (10):852-9
  • Waugh DJ, Wilson C. The interleukin-8 pathway in cancer. Clin Cancer Res. 2008 Nov 01;14 (21):6735-41
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