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Neuroglobin Human, Mouse Monoclonal Antibody, Clone: 13C8

  • Regulatory status:RUO
  • Type:Monoclonal Antibody
  • Other names:NGB
  • Species:Human
Cat. No. Size Price
1 pc / 2 - 5 pcs / 6+ pcs

RD182043100-C8 0.1 mg $300 / $266 / On request
PubMed Product Details
Technical Data


Monoclonal Antibody



Antibodies Applications

Source of Antigen

E. coli








The antibody is a mouse monoclonal antibody against recombinant Human Neuroglobin.

Amino Acid Sequence

The immunization antigen (17 kDa) is a protein containing 150 AA of recombinant Human Neuroglobin and one extra AA, N-terminal methionin.


The amino acid sequence of the recombinant Human Neuroglobin is 100% homologous with the amino acid sequence of the Human Neuroglobin

Species Reactivity

Human. Not yet tested in other species.

Purification Method

Affinity chromatography on a column with immobilized protein G.

Antibody Content

0.1 mg (determined by BCA method, BSA was used as a standard)


The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. 


Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


The lyophilized antibody remains stable and fully active until the expiry date when stored at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody SDS PAGE – to determine purity of the antibody BCA - to determine quantity of the antibody


This product is for research use only.


Research topic

Neural tissue markers


Neuroglobin, 151 amino acid reside protein, mainly expressed in vertebrate brain and retina, is a recently identified member of the globin superfamily. Augmenting O(2) supply, neuroglobin promotes survival of neurons upon hypoxic injury, potentially limiting brain damage. Moreover, neuroglobin may be a novel oxidative stress-responsive sensor for signal transduction in the brain. Neuroglobin expression is increased by neuronal hypoxia in vitro and focal cerebral ischemia in vivo, and neuronal survival after hypoxia is reduced by inhibiting neuroglobin expression with an antisense oligodeoxynucle­otide and enhanced by neuroglobin overexpression

Product References (5)


  • Xun Y, Li Z, Tang Y, Yang M, Long S, Shu P, Li J, Xiao Y, Tang F, Wei C, Liu N, Xiang S. Neuroglobin Regulates Wnt/β-Catenin and NFκB Signaling Pathway through Dvl1. Int J Mol Sci. 2018 Jul 23;19(7):2133. doi: 10.3390/ijms19072133. PubMed PMID: 30041403. PubMed CentralPMCID: PMC6073292. See more on PubMed
  • Emara M, Salloum N, Allalunis-Turner J. Expression and hypoxic up-regulation of neuroglobin in human glioblastoma cells. Mol Oncol. 2009 Feb;3(1):45-53. doi: 10.1016/j.molonc.2008.11.002. Epub 2008 Dec 12. PubMed PMID: 19383366. PubMed CentralPMCID: PMC5527870. See more on PubMed
  • Ostojić J, Grozdanić SD, Syed NA, Hargrove MS, Trent JT 3rd, Kuehn MH, Kwon YH, Kardon RH, Sakaguchi DS. Patterns of distribution of oxygen-binding globins, neuroglobin and cytoglobin in human retina. Arch Ophthalmol. 2008 Nov;126(11):1530-6. doi: 10.1001/archopht.126.11.1530. PubMed PMID: 19001220. See more on PubMed
  • Ostojic J, Grozdanic S, Syed NA, Hargrove MS, Trent JT 3rd, Kuehn MH, Kardon RH, Kwon YH, Sakaguchi DS. Neuroglobin and cytoglobin distribution in the anterior eye segment: a comparative immunohistochemical study. J Histochem Cytochem. 2008 Sep;56(9):863-72. doi: 10.1369/jhc.2008.951392. Epub 2008 Jun 23. PubMed PMID: 18574250. PubMed CentralPMCID: PMC2516955. See more on PubMed
  • Ostojić J, Sakaguchi DS, de Lathouder Y, Hargrove MS, Trent JT 3rd, Kwon YH, Kardon RH, Kuehn MH, Betts DM, Grozdanić S. Neuroglobin and cytoglobin: oxygen-binding proteins in retinal neurons. Invest Ophthalmol Vis Sci. 2006 Mar;47(3):1016-23. doi: 10.1167/iovs.05-0465. PubMed PMID: 16505036. See more on PubMed
Summary References (5)

References to Neuroglobin

  • Garry DJ, Mammen PP. Neuroprotection and the role of neuroglobin. Lancet. 2003 Aug 2;362 (9381):342-3
  • Mammen PP, Shelton JM, Goetsch SC, Williams SC, Richardson JA, Garry MG, Garry DJ. Neuroglobin, a novel member of the globin family, is expressed in focal regions of the brain. J Histochem Cytochem. 2002 Dec;50 (12):1591-8
  • Pesce A, Dewilde S, Nardini M, Moens L, Ascenzi P, Hankeln T, Burmester T, Bolognesi M. Human brain neuroglobin structure reveals a distinct mode of controlling oxygen affinity. Structure. 2003 Sep;11 (9):1087-95
  • Sun Y, Jin K, Mao XO, Zhu Y, Greenberg DA. Neuroglobin is up-regulated by and protects neurons from hypoxic-ischemic injury. Proc Natl Acad Sci U S A. 2001 Dec 18;98 (26):15306-11
  • Sun Y, Jin K, Peel A, Mao XO, Xie L, Greenberg DA. Neuroglobin protects the brain from experimental stroke in vivo. Proc Natl Acad Sci U S A. 2003 Mar 18;100 (6):3497-500
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