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Manufactured by BioVendor

Phosphorylated Neurofilament H Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:pNF-H
  • Species:Human
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Cat. No. Size Price

RD191138300R 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA, HRP-labelled antibody


Serum, Cerebrospinal fluid, Tissue extract, Plasma


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

62.5–4000 pg/ml

Limit of Detection

23.5 pg/ml

Intra-assay (Within-Run)

n = 8; CV = 4.5%

Spiking Recovery

CSF sample: 98.8%
Serum sample: 95.8%

Dilution Linearity

CSF sample: 106.1%
Serum sample: 105.5%



  • It is intended for research use only.
  • The total assay time is less than 4 hours.
  • The kit measures pNF-H in serum, plasma, cerebrospinal fluid (CSF) and tissue samples
  • Assay format is 96 wells.
  • Standard and Quality Controls are human brain extract based. No animal sera are used.
  • Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Neural tissue markers, Oncology

Product References (29)


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Summary References (7)

References to Phosphorylated Neurofilament H

  • Anderson KJ, Scheff SW, Miller KM, Roberts KN, Gilmer LK, Yang C, Shaw G. The phosphorylated axonal form of the neurofilament subunit NF-H (pNF-H) as a blood biomarker of traumatic brain injury. J Neurotrauma. 2008 Sep;25 (9):1079-85
  • Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Iqbal K, Grundke-Iqbal I, Wang JZ. Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett. 2002 Mar 8;320 (3):156-60
  • Lewis SB, Wolper RA, Miralia L, Yang C, Shaw G. Detection of phosphorylated NF-H in the cerebrospinal fluid and blood of aneurysmal subarachnoid hemorrhage patients. J Cereb Blood Flow Metab. 2008 Jun;28 (6):1261-71
  • Petzold A. Neurofilament phosphoforms: surrogate markers for axonal injury, degeneration and loss. J Neurol Sci. 2005 Jun 15;233 (1-2):183-98
  • Petzold A, Rejdak K, Plant GT. Axonal degeneration and inflammation in acute optic neuritis. J Neurol Neurosurg Psychiatry. 2004 Aug;75 (8):1178-80
  • Petzold A, Shaw G. Comparison of two ELISA methods for measuring levels of the phosphorylated neurofilament heavy chain. J Immunol Methods. 2007 Jan 30;319 (1-2):34-40
  • Shaw G, Yang C, Ellis R, Anderson K, Parker Mickle J, Scheff S, Pike B, Anderson DK, Howland DR. Hyperphosphorylated neurofilament NF-H is a serum biomarker of axonal injury. Biochem Biophys Res Commun. 2005 Nov 4;336 (4):1268-77
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