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QuickZyme Sensitive Tissue Hydroxyproline Kit

  • Regulatory status:RUO
  • Type:Colorimetric assay
  • Species:Multispecies
Cat. No. Size Price

Discount QZBTISHYP1 96 wells (1 kit) $684,8
New QZBTISHYP2 2 x 96 wells (1 kit) $1120,35
New QZBTISHYP5 5 x 96 wells (1 kit) $2038,58
PubMed Product Details
Technical Data


Colorimetric assay


Now hydroxyproline can be easily determined in small tissue samples with low collagen levels!

Dysregulation in collagen metabolism may result in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Therefore measurement of collagen production is important in many disease related studies.

Hydroxyproline is a non-proteinogenic amino acid, which in mammals occurs in elastin and collagen. Its presence is mainly limited to the triple helix of collagen, where it leads to increased triple helix stability. Hydroxyproline is formed post-translationally from specific proline residues by action of the enzyme prolylhydroxylase. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present.

The QuickZyme Sensitive Tissue Hydroxyproline assay is a modification of the method described by Prockop and Udenfriend (Anal. Biochem.,1960, 1: 228-239). The assay measures the total amount of hydroxyproline present in the sample. If this hydroxyproline is obtained upon hydrolysis of collagen, it represents all the types of collagen present in the sample without discriminating between the types of collagen and between procollagen, mature collagen and collagen degradation products.

The QuickZyme Sensitive Tissue Hydroxyproline assay kit is the only hydroxyproline-based assay which shows no matrix effect and no need for further dilution of the hydrolysate enabling measurement in small samples with low hydroxyproline concentrations. In addition, no drying of hydrolysate is needed, resulting in a fast (<2 hrs), sensitive and easy (96-well plate format) assay.


Tissue, Tissue homogenates

Sample Requirements

5 - 35 μl


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Unopened kit: Store at room temperature in the dark. Do not use kit components past the kit expiration date.

Opened kit / reconstituted reagents: The opened standard, assay buffer and enhancer solution should be stored light protected at 4°C. The other opened reagents should be stored light protected at RT and are stable for at least 1 month. The reconstituted detection reagent (A+B) should be used on the day of reconstitution.

Calibration Range

0 - 300 µM

Limit of Detection

2.4 µM (0.3 µg/ml)


NOTE: This kit does not contain tubes and a protocol for collagen hydrolysis. If you need these items please order the QuickZyme Sensitive Tissue Collagen Assay



  • Quantitative measurement of hydroxyproline

  • Optimized for tissues with low collagen or hydroxyproline content (such as liver) due to absence of matrix effects

  • Samples: tissue homogenates, tissues

  • Range: 6 to 300 μM. Sensitivity: 2.4 µM (0.3 µg/ml)

  • Uses hydroxyproline as standard

  • Ease-of-use: Equivalent to ELISA; Colorimetric read out

  • Storage at room temperature

  • NOTE: This kit does not contain tubes and a protocol for collagen hydrolysis. If you need these items please order the QuickZyme Sensitive Tissue Collagen Assay

Research topic

Extracellular matrix


Collagens are the most abundant proteins in mammals. The collagen family comprises 28 members that contain at least one triple-helical domain. Collagens are deposited in the extracellular matrix where most of them form supramolecular assemblies. Four collagens are type II membrane proteins that also exist in a soluble form released from the cell surface by shedding. Collagens play structural roles and contribute to mechanical properties, organization, and shape of tissues. They interact with cells via several receptor families and regulate their proliferation, migration, and differentiation. Some collagens have a restricted tissue distribution and hence specific biological functions.

Summary References (1)

References to Collagen

  • Ricard-Blum S. The collagen family. Cold Spring Harb Perspect Biol. 2011 Jan 1;3(1):a004978. doi: 10.1101/cshperspect.a004978. PMID: 21421911; PMCID: PMC3003457. See more on PubMed
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