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QuickZyme Soluble Collagen Assay Kit

  • Regulatory status:RUO
  • Type:Colorimetric assay
  • Species:Multispecies
Cat. No. Size Price

Discount QZBCOL1-1 96 wells (1 kit) $587,03
New QZBCOL1-2 2 x 96 wells (1 kit) $964,43
New QZBCOL1-5 5 x 96 wells (1 kit) $1692,08
PubMed Product Details
Technical Data


Colorimetric assay


Measure all types of fibrillary collagen in a fast and simple 96-well plate format!

Collagen is one of the main components of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Therefore measurement of collagen production is important in many disease related studies.

The QuickZyme collagen assay is based on binding of the dye Sirius Red to collagen. Following binding of the dye, the collagen-dye complex precipitates, resulting in a coloured pellet. This colour can be released in an alkaline solution.

The assay measures various types of fibrillar collagen. The most abundant collagen types are collagen type I (skin, bone, vessels, connective tissue), type II (cartilage) and type III (skin, bone, connective tissue).

The assay is specific for collagen. After heating at 50°C the signal is lost, since the assay does not recognize denatured collagen (gelatin). The assay can be used to analyze soluble collagen or collagen that is solubilized by acetic acid alone or in combination with pepsin. The assay is intended for collagen analysis in conditioned cell culture media and cellular extracts. The assay is not affected by serum up to at least 10%.


Cellular extracts, Tissue homogenates, Tissue culture supernatants

Sample Requirements

10 - 140 µl


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Unopened kit: Store at 4°C. Do not use kit components past kit expiration date.

Opened kit / reconstituted reagents:

  • Collagen standard dissolved in 20 mM HAc: store at 4°C up to 1 month

  • Dye solution: store at 4°C up to 1 month

  • Dilution buffer: store at -20°C up to 6 months

  • Wash buffer: store at -20°C up to 6 months

  • Detection solution: store either at 4°C or room temperature up to 1 month

Calibration Range

0 - 10 µg/well

Limit of Detection

0.69 ng/ml



  • Measures all types of fibrillar collagen, species independent

  • Measures soluble or solubilized collagens

  • Samples: tissue culture supernatants, cellular extracts and tissue homogenates

  • 96-well plate format

  • Quantitative

  • Range/sensitivity: 0.5 to 10 μg/ml. Sensitivity: 0.69 ng/ml

  • Ease-of-use: Equivalent to ELISA

  • Assay time < 3 hours

  • Store at 4°C

Research topic

Extracellular matrix


Collagens are the most abundant proteins in mammals. The collagen family comprises 28 members that contain at least one triple-helical domain. Collagens are deposited in the extracellular matrix where most of them form supramolecular assemblies. Four collagens are type II membrane proteins that also exist in a soluble form released from the cell surface by shedding. Collagens play structural roles and contribute to mechanical properties, organization, and shape of tissues. They interact with cells via several receptor families and regulate their proliferation, migration, and differentiation. Some collagens have a restricted tissue distribution and hence specific biological functions.

Summary References (1)

References to Collagen

  • Ricard-Blum S. The collagen family. Cold Spring Harb Perspect Biol. 2011 Jan 1;3(1):a004978. doi: 10.1101/cshperspect.a004978. PMID: 21421911; PMCID: PMC3003457. See more on PubMed
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