Sandwich ELISA, Biotin-labelled antibody
Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Limit of Detection
n = 8; CV = 5.9%
n = 5; CV = 7.6%
- bovine Non-detectable
- cat Non-detectable
- dog Non-detectable
- goat Non-detectable
- hamster Non-detectable
- horse Yes (recommended dilution 1:6)
- rabbit Non-detectable
- rat Non-detectable
- sheep Non-detectable
- chicken Not tested
- human Yes
- monkey Yes (recommended dilution 1:3)
- mouse Yes (recommended dilution 1:3)
- pig Yes (recommended dilution 1:3)
- European Union: for in vitro diagnostic use
- Rest of the world: for research use only!
- The total assay time is less than 4 hours
- The kit measures total resistin in serum and plasma (EDTA, citrate, heparin)
- Assay format is 96 wells
- Quality Controls are human serum based
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Diabetology - Other Relevant Products, Energy metabolism and body weight regulation
Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteinerich
secreted proteins which is termed the RELM family, and is also described as ADSF
(Adipose Tissue-Specific Secretory Factor) or FIZZ3 (Found in Inflammatory Zone). Human
resistin contains 108 amino acids as a prepeptide, and its hydrophobic signal peptide is
cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting
of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.
Much of the early investigations about the resistin molecule are based on the mouse model.
Resistin, produced and secreted primarily by adipocytes in mice, acts on skeletal muscle
myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin.
Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate
glucose uptake. Other studies have shown that mouse resistin increases during the
differentiation of adipocytes, but it also seems to inhibit adipogenesis.
Compared to the mouse model, human adipogenic differentiation is likely to be associated with
a down regulation of resistin gene expression. On the other hand, resistin was found to be
expressed at high levels in human monocytes, macrophages and bone marrow.
Recent investigations have shown that human resistin is correlated with metabolic syndrome
and obesity-related disorders. Malo et al. have reported that resistin levels are positively
associated with waist circumference, tumor necrosis factor-a, and insulin resistance assessed
by the homeostasis model, and inversely correlated with total cholesterol, HDL cholesterol, and
LDL cholesterol. Moreover, Sadhasiv et al. found a positive correlation of SAT (subcutaneous
adipose tissue) resistin mRNA expression with serum resistin, BMI and insulin resistance
Based on the above reports human resistin might be an important marker that acts as the link
between obesity and insulin resistance. Resistin can play a role also in inflammation processes
and in atherosclerosis.
Clinical use and areas of investigation: Energy metabolism and body weight regulation, Metabolic syndrome, Inflammation, Atherosclerosis