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Manufactured by BioVendor

S100A7A Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:S100-A15, S100 calcium-binding protein A7A, S100 calcium-binding protein A7-like 1,S100 calcium-binding protein A15
  • Species:Human
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Cat. No. Size Price


RD172213100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 109 AA. MW: 12.3 kDa (calculated). UniProtKB acc.no. Q86SG5. N-Terminal His-tag, 9 extra AA (highlighted).

Amino Acid Sequence

MKHHHHHHASNTQAERSIIGMIDMFHKYTGRDGKIEKPSLLTMMKENFPNFLSACDKKGIHYLATVFEKKDKNEDKKIDFSEFLSLLGDIAADYHKQSHGAAPCSGGSQ

Source

E. coli

SDS-PAGE Gel

14% SDS-PAGE separation of Human S100A15 protein
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and boiled sample, 5μg / lane
3. non-reduced and non-boiled sample, 5μg / lane

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely.Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Applications

Western blotting

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Summary

Research topic

Oncology, Others

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