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Manufactured by BioVendor

S100B Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Species:Human
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Cat. No. Size Price


RD192090100R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Cerebrospinal fluid

Sample Requirements

25 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

10–320 pg/ml

Limit of Detection

The measurement range of the assay is 10 – 320 pg/ml (final concentration in a well after dilution).

Intra-assay (Within-Run)

n = 8; CV = 2.0 %

Inter-assay (Run-to-Run)

n = 4; CV = 5.9 %

Spiking Recovery

102.5 %

Dilution Linearity

111.6 %

Crossreactivity

  • bovine Yes
  • cat Yes
  • dog Non-detectable
  • horse Non-detectable
  • goat Yes
  • hamster Yes
  • mouse Non-detectable
  • pig Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • monkey Yes
  • human Yes
  • rabbit Yes
  • rat Yes
Summary

Features

  • It is intended for research use only
  • The total assay time is less than 5 hours
  • The kit measures S100B protein in serum and cerebrospinal fluid
  • Assay format is 96 wells
  • Quality Controls are human serum based. Animal serum is used for Master Standard and for Dilution Buffer preparation
  • Standard is native protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Neural tissue markers, Oncology

Summary

S-100B protein is a member of highly homologous acidic calcium and zinc binding proteins family (S100 family) that possess two EF-hand motifs. S100B is a bb homodimer with the molecular weight 21 kDa. S100B is abundant in the nervous system where it is predominantly expressed in astrocytes, oligodendrocytes and Schwann cells. S100B is released from brain tissue into cerebrospinal fluid (CSF) and blood following brain damage of different origin, i.e., brain infarction, intracerebral or subarachnoidal hemorrhage, neurotrauma and large intracerebral tumors. It is documented a relationship between degree of cell damage and S100B levels in CSF. Also patients with progressive melanoma disease show elevated serum concentrations of S100B.

Product References (41)

References

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Summary References (15)

References to S100B

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  • Sanchez-Juan P et al: CSF tests in the differential diagnosis of Creutzfeldt-Jakob disease. Neurology. 2006 Aug 22;67(4):637–43.
  • Steiner J et al: Increased cerebrospinal fluid and serum levels of S100B in first-onset schizophrenia are not related to a degenerative release of glial fibrillar acidic protein, myelin basic protein and neurone-specific enolase from glia or neurones. J Neurol Neurosurg Psychiatry. 2006 Nov;77(11):1284–7.
  • Steiner J et al: Evidence for a wide extra-astrocytic distribution of S100B in human brain. BMC Neurosci. 2007 Jan 2;8:2.
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