Sandwich ELISA, Biotin-labelled antibody
Serum, Cerebrospinal fluid
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Limit of Detection
The measurement range of the assay is 10 – 320 pg/ml (final concentration in a well after dilution).
n = 8; CV = 2.0 %
n = 4; CV = 5.9 %
- bovine Yes
- cat Yes
- dog Non-detectable
- horse Non-detectable
- goat Yes
- hamster Yes
- mouse Non-detectable
- pig Non-detectable
- sheep Non-detectable
- chicken Not tested
- monkey Yes
- human Yes
- rabbit Yes
- rat Yes
- It is intended for research use only
- The total assay time is less than 5 hours
- The kit measures S100B protein in serum and cerebrospinal fluid
- Assay format is 96 wells
- Quality Controls are human serum based. Animal serum is used for Master Standard and for Dilution Buffer preparation
- Standard is native protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Neural tissue markers, Oncology
S-100B protein is a member of highly homologous acidic calcium and zinc binding proteins family (S100 family) that possess two EF-hand motifs. S100B is a bb homodimer with the molecular weight 21 kDa. S100B is abundant in the nervous system where it is predominantly expressed in astrocytes, oligodendrocytes and Schwann cells. S100B is released from brain tissue into cerebrospinal fluid (CSF) and blood following brain damage of different origin, i.e., brain infarction, intracerebral or subarachnoidal hemorrhage, neurotrauma and large intracerebral tumors. It is documented a relationship between degree of cell damage and S100B levels in CSF. Also patients with progressive melanoma disease show elevated serum concentrations of S100B.