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sHLA-G ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:soluble HLA-G
  • Species:Human
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Cat. No. Size Price


RD194070100R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Plasma-EDTA, Amniotic fluid, Cell culture supernatant

Sample Requirements

25 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

3.91–125 Units/ml

Limit of Detection

0.6 Units/ml

Intra-assay (Within-Run)

n = 8; CV = 4.2%

Inter-assay (Run-to-Run)

n = 11; CV = 7.0%

Spiking Recovery

91,20%

Dilutation Linearity

99,40%

Summary

Features

  • It is intended for research use only
  • The total assay time is about 20 hours
  • The kit measures shedded HLA-G1 and HLA-G5 in EDTA plasma, amniotic fluid, or cell culture supernatant
  • Calibrator is human native protein
  • Assay format is 96 wells
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Immune Response, Infection and Inflammation, Reproduction, Transplantation

Summary

HLA-G differs from the other MHC class I genes by its low polymorphism and alternative splicing that generates seven HLA-G proteins, whose tissue-distribution is restricted to normal fetal and adult tissues that display a tolerogeneic function toward both innate and acquired immune cells. Soluble HLA-G is an immunosuppressive molecule inducing apoptosis of activated CD8(+) T cells and down-modulating CD4(+) T cell proliferation. Recently, using specific ELISA to analyse the presence of sHLA-G molecules in culture supernatants of early embryos obtained by in vitro fertilization (IVF) before transfer, several reports demonstrated that positive embryo implantations occurred with embryo secreting sHLA-G molecules. These breakthrough results indicate that sHLA-G ELISA can be a useful biochemical assay in addition to embryo morphology in embryo selection for transfer in IVF treatment if there are other embryos with the same morphology. Furthermore, monitoring of sHLA-G in amniotic fluid and plasma of pregnant women may have an important prognostic value to recognize pathological situations. Other interesting observations suggest that HLA-G molecules seem to be directly involved in transplant acceptation, and their analysis should be taken into consideration when monitoring transplant-patients status. In addition, soluble HLA-G plasma levels are increased in lyphoproliferative disorders or in patients suffering from malignant melanoma, glioma, breast and ovarian cancer.

References to Product

References

  • Chen CH, Liao HT, Chen HA, Liu CH, Liang TH, Wang CT, Tsai CY, Chou CT. Human leukocyte antigen-G in ankylosing spondylitis and the response after tumour necrosis factor-alpha blocker therapy. Rheumatology (Oxford). 2010 Feb;49 (2):264-70
  • Chen XY, Yan WH, Lin A, Xu HH, Zhang JG, Wang XX. The 14 bp deletion polymorphisms in HLA-G gene play an important role in the expression of soluble HLA-G in plasma. Tissue Antigens. 2008 Oct;72 (4):335-41
  • Ciprandi G, Colombo BM, Contini P, Cagnati P, Pistorio A, Puppo F, Murdaca G. Soluble HLA-G and HLA-A,-B,-C serum levels in patients with allergic rhinitis. Allergy. 2008 Oct;63 (10):1335-8
  • Coulam C.B., Roussev R.G. Soluble HLA G concentrations in culture media from in vitro fertilized human embryos are predicitive of implantation potential. American Journal of Reproducti. 2005;:53:279-309
  • Coulam CB, Roussev RG. Soluble HLA G Concentrations in Culture Media From In Vitro Fertilized Human Embryos are Predicative of Implantation Potential. Fertility and Sterility . 84(1):S447-S448 (2005)
  • Coulam CB, Roussev RG. Soluble HLA G concentrations in culture media from in vitro fertilized human embryos are predicitive of implantation potential. American Journal of Reproductive Immunology . 53:279-309 (2005)
  • Coulam CB, Roussev RG, Lerner S, Zlatopolsky Z, Ilkevitch Y, Tur-Kaspa I. How to predict implantation? No correlation between embryonic aneuploidy and soluble human leukocyte antigen G-concentrations. Fertil Steril. 2009 Jun;91 (6):2408-13
  • Desai N, Filipovits J, Goldfarb J. Secretion of soluble HLA-G by day 3 human embryos associated with higher pregnancy and implantation rates: assay of culture media using a new ELISA kit. Repro Biomed Online . Aug;13(2):272-7 (2006)
  • Desai N, Filipovits J, Singh P, Beman M, Goldfarb J. Assessment of soluble HLA-G levels in day 3 human embryo culture media using a new ELISA kit: Is there a relationship to embryo quality, development and implantation potential?. Fertility & Sterility . Vol. 84, Suppl 1 (S281-S282), September 2005
  • Di Cristofaro J, El Moujally D, Agnel A, Mazieres S, Cortey M, Basire A, Chiaroni J, Picard C. HLA-G haplotype structure shows good conservation between different populations and good correlation with high, normal and low soluble HLA-G expression. Hum Immunol. 2013 Feb;74 (2):203-6
  • Dunker K, Schlaf G, Bukur J, Altermann WW, Handke D, Seliger B. Expression and regulation of non-classical HLA-G in renal cell carcinoma. Tissue Antigens. 2008 Aug;72 (2):137-48
  • Eidukaite A, Tamosiunas V. Soluble HLA-G in the peritoneal fluid of women with endometriosis. Fertil Steril . May 15 (2007)
  • Giannopoulos K, Schmitt M, Kowal M, Wlasiuk P, Bojarska-Junak A, Rolinski J, Dmoszynska A. The significance of soluble HLA-G plasma levels as well as messenger HLA-G for B-cell chronic lymphocytic leukemia (B-CLL). Leuk Res. 2008 Dec;32 (12):1815-9
  • Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A. Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation. PLoS One. 2011;6 (5):e19988
  • Heidari MH, Novin MG, Ahmadi F, Khorshid M, Najafi T, Molouki A, Salehpour S, Bandehpour M, Heidari M, Sato E. Increase in concentration of soluble HLA-G in high-quality embryos after intracytoplasmic sperm injection. Anat Cell Biol. 2011 Dec;44 (4):331-6
  • Ivanova-Todorova E, Mourdjeva M, Kyurkchiev D, Bochev I, Stoyanova E, Dimitrov R, Timeva T, Yunakova M, Bukarev D, Shterev A, Tivchev P, Kyurkchiev S. HLA-G expression is up-regulated by progesterone in mesenchymal stem cells. Am J Reprod Immunol. 2009 Jul;62 (1):25-33
  • Jee BC, Suh CS, Kim SH, Moon SY. Soluble human leukocyte antigen G level in fluid from single dominant follicle and the association with oocyte competence. Yonsei Med J. 2011 Nov;52 (6):967-71
  • Kronsteiner B, Peterbauer-Scherb A, Grillari-Voglauer R, , Redl H, Gabriel C, van Griensven M, Wolbank S. Human mesenchymal stem cells and renal tubular epithelial cells differentially. Cellular Immunology. 2010;
  • Kusanovic JP, Romero R, Jodicke C, Mazaki-Tovi S, Vaisbuch E, Erez O, Mittal P, Gotsch F, Chaiworapongsa T, Edwin SS, Pacora P, Hassan SS. Amniotic fluid soluble human leukocyte antigen-G in term and preterm parturition, and intra-amniotic infection/inflammation. J Matern Fetal Neonatal Med. 2009 Dec;22 (12):1151-66
  • Laskowska M, Laskowska K,Oleszczuk K. Umbilical Serum sHLA-G Levels in Preeclamptic Pregnancies with and without Intrauterine Fetal Growth Restriction: A Comparison with Normotensive Pregnancies with Isolated IUGR and Healthy Controls. International Scholarly Resear. 20 July 2011;2011:6
  • Le Maux A, Noel G, Birebent B, Grosset JM, Vu N, De Guibert S, Bernard M, Semana G, Amiot L. Soluble human leucocyte antigen-G molecules in peripheral blood haematopoietic stem cell transplantation: a specific role to prevent acute graft-versus-host disease and a link with regulatory T cells. Clin Exp Immunol. 2008 Apr;152 (1):50-6
  • Levitsky J, Miller J, Wang E, Rosen A, Flaa C, Abecassis M, Mathew J, Tambur A. Immunoregulatory profiles in liver transplant recipients on different immunosuppressive agents. Hum Immunol. 2009 Mar;70 (3):146-50
  • Mach P, Blecharz P, Basta P, Marianowski P, Skret-Magierlo J, Kojs Z, Grabiec M, Wicherek L. Differences in the soluble HLA-G blood serum concentration levels in patients with ovarian cancer and ovarian and deep endometriosis. Am J Reprod Immunol. 2010 May;63 (5):387-95
  • Mariconti M, Meroni V, Badulli C, Brunetti E, Tinelli C, De Silvestri A, Tamarozzi F, Genco F, Casulli A, Martinetti M. Correlation of serum sHLA-G levels with cyst stage in patients with cystic echinococcosis: is it an immune-evasion strategy?. Parasite Immunol. 2016 Apr 27;
  • Masson E, Vidal C, Deschamps M, Bongain S, Thevenin C, Dupont I, Rietmulher D, Pouthier F, Mongaillard G, Chabod J, Ferrand C, Tiberghien P, Rebibou JM. Incidence and risk factors of anti-HLA immunization after pregnancy. Hum Immunol. 2013 Aug;74 (8):946-51
  • McCormick J, Whitley GS, Le Bouteiller P, Cartwright JE. Soluble HLA-G regulates motility and invasion of the trophoblast-derived cell line SGHPL-4. Hum Reprod. 2009 Jun;24 (6):1339-45
  • Nicodemus-Johnson J, Laxman B, Stern RK, Sudi J, Tierney CN, Norwick L, Hogarth DK, McConville JF, Naureckas ET, Sperling AI, Solway J, Krishnan JA, Nicolae DL, White SR, Ober C. Maternal asthma and microRNA regulation of soluble HLA-G in the airway. J Allergy Clin Immunol. 2013 Jun;131 (6):1496-503
  • Raicevic G, Rouas R, Najar M, Stordeur P, Boufker HI, Bron D, Martiat P, Goldman M, Nevessignsky MT, Lagneaux L. Inflammation modifies the pattern and the function of Toll-like receptors expressed by human mesenchymal stromal cells. Hum Immunol. 2010 Mar;71 (3):235-44
  • Rajasekarana A, Shivakumar V, Kalmady S V, Narayanaswamy J C,Subbana M, Venugopal D, Amaresha A C, Venkatasubramanian G, Debnath M. The impact of IL-10 polymorphisms and sHLA-G levels on the risk of schizophrenia. Asian Journal of Psychiatry. 27 June 2016;
  • Roman A, Rodriguez M, Herraiz MA, Jorda J, Cervera I, Penaloza J, Vidart JA, Martinez-Laso J. Heterogeneous expression of HLA-G1, -G2, -G5, -G6, and -G7 in myeloid and plasmacytoid dendritic cells isolated from umbilical cord blood. Hum Immunol. 2009 Feb;70 (2):104-9
  • Roussev R.G., Acacio B., Parrett S., Romineb H., Coulam C.B. Soluble HLA-G as potential candidate to help morphology grading score in embryo selection for IVF/ET. Journal of Reproductive Immuno. 2009;:81, 113–175
  • Roussev RG, Suchanek M. Soluble HLA-G in culture medium from individually cultured human embryos is necessary but not essential for successful implantation in IVF/ET. -2005
  • Shaikly VR, Morrison IE, Taranissi M, Noble CV, Withey AD, Cherry RJ, Blois SM, Fernandez N. Analysis of HLA-G in maternal plasma, follicular fluid, and preimplantation embryos reveal an asymmetric pattern of expression. J Immunol. 2008 Mar 15;180 (6):4330-7
  • Sipak-Szmigiel O, Włodarski P, Ronin-Walknowska E, Niedzielski A, Karakiewicz B, Słuczanowska-Głąbowska S, Laszczyńska M, Malinowski W. Serum and peritoneal fluid concentrations of soluble human leukocyte antigen, tumor necrosis factor alpha and interleukin 10 in patients with selected ovarian pathologies. J Ovarian Res. 2017 Apr 4;10(1):25. doi: 10.1186/s13048-017-0320-9
  • Solini A, Muscelli E, Stignani M, Melchiorri L, Santini E, Rossi C, Astiarraga BD, Rizzo R, Baricordi OR. Soluble human leukocyte antigen-g expression and glucose tolerance in subjects with different degrees of adiposity. J Clin Endocrinol Metab. 2010 Jul;95 (7):3342-6
  • Steinborn A, Varkonyi T, Scharf A, Bahlmann F, Klee A, Sohn C. Early detection of decreased soluble HLA-G levels in the maternal circulation predicts the occurrence of preeclampsia and intrauterine growth retardation during further course of pregnancy. Am J Reprod Immunol . Apr;57(4):277-86 (2007)
  • Tahan F, Eke Gungor H, Akar HH, Saraymen B. Increased Levels of Plasma Soluble Human Leukocyte Antigen G in Persistent Wheezy Infants. Pediatr Int. 2016 Nov 23;
  • Tahan F, Patiroglu T. Plasma soluble human leukocyte antigen G levels in asthmatic children. Int Arch Allergy Immunol . 141(3):213-6 (2006)
  • Wang F, Wen Z, Li H, Yang Z, Zhao X, Yao X. Human leukocyte antigen-G is expressed by the eutopic and ectopic endometrium of adenomyosis. Fertil Steril. 2008 Nov;90 (5):1599-604
  • Wiendl H, Feger U, Mittelbronn M, Jack C, Schreiner B, Stadelmann C, Antel J, Brueck W, Meyermann R, Bar-Or A, Kieseier BC, Weller M. Expression of the immune-tolerogenic major histocompatibility molecule HLA-G in multiple sclerosis: implications for CNS immunity. Brain. 2005 Nov;128 (Pt 11):2689-704
  • Wiendl H, Mitsdoerffer M, Hofmeister V, Wischhusen J, Bornemann A, Meyermann R, Weiss EH, Melms A, Weller M. A functional role of HLA-G expression in human gliomas: an alternative strategy of immune escape. J Immunol . May 1;168(9):4772-80 (2002)
  • Wlasiuk P, Tomczak W, Zajac M, Dmoszynska A, Giannopoulos K. Total expression of HLA-G and TLR-9 in chronic lymphocytic leukemia patients. Hum Immunol. 2013 Aug 29;
  • Zhang JB, Wang ZY, Chen J, Wu XD, Zhou B, Yie SM. The expression of human leukocyte antigen G (HLA-G) is associated with sacroiliitis stages of ankylosing spondylitis. Immunol Lett. 2013 May;152 (2):121-5
  • Zheng J, Xu C, Chu D, Zhang X, Li J, Ji G, Hong L, Feng Q, Li X, Wu G, Du J, Zhao Q. Human leukocyte antigen G is associated with esophageal squamous cell carcinoma progression and poor prognosis. Immunol Lett. 2014 Apr 21;
  • Zhu CB, Wang CX, Zhang X, Zhang J, Li W. Serum sHLA-G levels: a useful indicator in distinguishing colorectal cancer from benign colorectal diseases. Int J Cancer. 2011 Feb 1;128 (3):617-22
References to Summary

References to HLA-G

  • Fujii T., Ishitani A., Geraghty D.E.: A soluble form of the HLA-G antigen is encoded by a messenger ribonucleic acid containing intron 4. J Immunol. 1994; 153: 5516–5524
  • Le Bouteiller P., Mallet V.: HLA-G and pregnancy. Rev Reprod. 1997; 2: 7–13
  • Blaschitz A., Lenfant F., Mallet V., Hartmann M., Bensussan A., Geraghty D.E., Le Bouteiller P., Dohr G.: Endothelial cells in chorionic fetal vessels of first trimester placenta express HLA-G. Eur Immunol. 1997; 27: 3380–3388
  • Chu W., Fant M.E., Geraghty D.E., Hunt J.S.: Soluble HLA-G in human placentas: synthesis in trophoblasts and interferon-gamma-activated macrophages but not placental fibroblasts. Hum Immunol. 1998; 59: 435–442
  • Moreau P., Paul P., Rouas-Freiss N., Kirszenbaum M., Dausset J., Carosella E.D.: Molecular and immunologic aspects of the nonclassical HLA class I antigen HLA-G: evidence for an important role in the maternal tolerance of the fetal allograft. Am J Reprod Immunol. 1998; 40: 136–144
  • Rebman V., Pfeiffer K., Passler M., Ferrone S., Maier S., Weiss E., Grosse-Wilde H.: Detection of soluble HLA-G molecules in plasma and amniotic fluid. Tissue Antigens. 1999; 53: 14–22
  • Hunt J.S., Jadhav L., Chu W., Geraghty D.E., Ober C.: Soluble HLA-G circulates in maternal blood during pregnancy. J Am Obstet Gynecol. 2000; 183: 682–688
  • Emmer P.M., Steegers E.A., van Lierop M.J., Steegers-Theunissen R.P., Loke Y.W., Joosten I.: Amniotic fluid soluble human leukocyte antigen G is markedly decreased in offspring with neural tube defects. Early Hum Dev. 2002; 66: 101–105
  • Fuzzi B., Rizzo R., Criscuoli L., Noci I., Melchiorri L., Scarselli B., Bencini E., Menicucci A., Baricordi O.R.: HLA-G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy. Eur J Immunol. 2002; 32: 311–315
  • Lila N., Amrein C., Guillemain R., Chevalier P., Latremouille C., Fabiani J.N., Dausset J., Carosella E.D., Carpentier A.: Human leukocyte antigen-G expression after heart transplantation is associated with a reduced incidence of rejection. Circulation. 2002; 105: 1949–1954
  • Carosella E.D., Moreau P., Le Maoult J., Le Discorde M., Dausset J., Rouas-Freiss N.: HLA-G molecules: from maternal-fetal tolerance to tissue acceptance. Adv Immunol. 2003; 81: 199–252
  • LeMaoult J., Le Discorde M., Rouas-Freiss N., Moreau P., Menier C., McCluskey J., Carosella E.D.: Biology and functions of human leukocyte antigen-G in health and sickness. Tissue Antigens. 2003; 62: 273–284
  • Le Bouteiller P., Legrand-Abravanel F., Solier C.: Soluble HLA-G1 at the materno-foetal interface—a review. Placenta. 2003; 24 Suppl A:S10–5
  • Steinborn A., Rebmann V., Scharf A., Sohn C., Grosse-Wilde H.: Placental abruption is associated with decreased maternal plasma levels of soluble HLA-G. J Clin Immunol. 2003; 23: 307–314
  • Le Bouteiller P., Pizzato N., Barakonyi A., Solier C.: HLA-G, pre-eclampsia, immunity and vascular events. J Reprod Immunol. 2003; 59: 219–234
  • Creput C., Durrbach A., Menier C., Guettier C., Samuel D., Dausset J., Charpentier B., Carosella E.D., Rouas-Freiss N.: Human leukocyte antigen-G (HLA-G) expression in biliary epithelial cells is associated with allograft acceptance in liver-kidney transplantation. J Hepatol. 2003; 39: 587–594
  • Sebti Y., Le Friec G., Pangault C., Gros F., Drenou B., Guilloux V., Bernard M., Lamy T., Fauchet R., Amiot L.: Soluble HLA-G molecules are increased in lymphoproliferative disorders. Hum Immunol. 2003; 64: 1093–1101
  • Rebmann V., Regel J., Stolke D., Grosse-Wilde H.: Secretion of sHLA-G molecules in malignancies. Semin Cancer Biol. 2003; 13: 371–377 sHLA-G ELISA (page 13 of 16) VERSION: 33 120607 15
  • Sher G., Keskintepe L., Nouriani M., Roussev R., Batzofin J.: Expression of sHLA-G in supernatants of individually cultured 46-h embryos: a potentially valuable indicator of 'embryo competency and IVF outcome. Reprod Biomed Online. 2004; 9: 74–78
  • Noci I., Fuzzi B., Rizzo R., Melchiorri L., Criscuoli L., Dabizzi S., Bi
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