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Manufactured by BioVendor

sHLA-G ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:soluble HLA-G
  • Species:Human
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Cat. No. Size Price


RD194070100R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Plasma-EDTA, Amniotic fluid, Cell culture supernatant

Sample Requirements

25 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

3.91–125 Units/ml

Limit of Detection

0.6 Units/ml

Intra-assay (Within-Run)

n = 8; CV = 4.2%

Inter-assay (Run-to-Run)

n = 11; CV = 7.0%

Spiking Recovery

91,20%

Dilution Linearity

99,40%

Summary

Features

  • It is intended for research use only
  • The total assay time is about 20 hours
  • The kit measures shedded HLA-G1 and HLA-G5 in EDTA plasma, amniotic fluid, or cell culture supernatant
  • Calibrator is human native protein
  • Assay format is 96 wells
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Immune Response, Infection and Inflammation, Reproduction, Transplantation

Summary

HLA-G differs from the other MHC class I genes by its low polymorphism and alternative splicing that generates seven HLA-G proteins, whose tissue-distribution is restricted to normal fetal and adult tissues that display a tolerogeneic function toward both innate and acquired immune cells. Soluble HLA-G is an immunosuppressive molecule inducing apoptosis of activated CD8(+) T cells and down-modulating CD4(+) T cell proliferation. Recently, using specific ELISA to analyse the presence of sHLA-G molecules in culture supernatants of early embryos obtained by in vitro fertilization (IVF) before transfer, several reports demonstrated that positive embryo implantations occurred with embryo secreting sHLA-G molecules. These breakthrough results indicate that sHLA-G ELISA can be a useful biochemical assay in addition to embryo morphology in embryo selection for transfer in IVF treatment if there are other embryos with the same morphology. Furthermore, monitoring of sHLA-G in amniotic fluid and plasma of pregnant women may have an important prognostic value to recognize pathological situations. Other interesting observations suggest that HLA-G molecules seem to be directly involved in transplant acceptation, and their analysis should be taken into consideration when monitoring transplant-patients status. In addition, soluble HLA-G plasma levels are increased in lyphoproliferative disorders or in patients suffering from malignant melanoma, glioma, breast and ovarian cancer.

Product References (42)

References

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Summary References (20)

References to HLA-G

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  • Sebti Y., Le Friec G., Pangault C., Gros F., Drenou B., Guilloux V., Bernard M., Lamy T., Fauchet R., Amiot L.: Soluble HLA-G molecules are increased in lymphoproliferative disorders. Hum Immunol. 2003; 64: 1093–1101
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  • Sher G., Keskintepe L., Nouriani M., Roussev R., Batzofin J.: Expression of sHLA-G in supernatants of individually cultured 46-h embryos: a potentially valuable indicator of 'embryo competency and IVF outcome. Reprod Biomed Online. 2004; 9: 74–78
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