Type
Sandwich ELISA, HRP-labelled antibody
Applications
Serum, Plasma, Cell culture supernatant
Sample Requirements
10 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store kit reagents between 2–8°C except control. Store lyophilized control at -20°C.
Calibration Curve
Calibration Range
6.25–100 ng/ml
Limit of Detection
2.2 ng/ml
Intra-assay (Within-Run)
CV = 4.1%
Inter-assay (Run-to-Run)
CV = 7.7%
Spiking Recovery
99%.
Dilution Linearity
93%
Research topic
Cell adhesion proteins
Summary
ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B-cells and dendritic cells in tonsils, lymph nodes and Peyer's patches. ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFNγ within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR.
The role of ICAM-1 as a disease marker has been demonstrated for a number of different indications and pathological situations. ICAM-1 upregulation in allergic airway inflammation is responsible for the recruitment of activated leukocytes and the pathogenesis of allergic rhinitis.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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