Type
Sandwich ELISA, HRP-labelled antibody
Description
The human sICAM-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sICAM-1 The human sICAM-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Applications
Serum, Urine, Amniotic fluid, Plasma, Cell culture supernatant, Bile
Sample Requirements
10 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store kit reagents between 2–8°C except control. Store lyophilized control at -20°C.
Calibration Curve
Calibration Range
6.3–100 ng/ml
Limit of Detection
2.2 ng/ml
Intra-assay (Within-Run)
CV = 4.1%
Inter-assay (Run-to-Run)
CV = 7.7%
Spiking Recovery
99%.
Dilution Linearity
93%
Specificity
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human sICAM-1 positive serum.There was no cross reactivity detected with sTNF-R (60 kDa), sTNF-R (80 kDa), IL-8/NAP-1, TNF, TNF, IFN, IFN2C, IFNω, IL-6, IL-2R, ELAM-1 and L-selectin.
Features
- RUO
- calibration range 6.3-100 ng/ml
- limit of detection 2.2 ng/ml
- intra-assay CV = 4.1%
- inter-assay CV = 7.7%
- lyophilized controls
Research topic
Cell adhesion proteins
Summary
Intercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-beta-complex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors. This family consists of the leukocyte adhesion glycoproteins LFA-1 which mediates lymphocyte adhesion, Mac-1 which mediates granulocyte adhesion and p150,95.
ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B-cells and dendritic cells in tonsils, lymph nodes and Peyer's patches. ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR.
The role of ICAM-1 as a disease marker has been demonstrated for a number of different indications and pathological situations.
Find documents for the lot
Example Instructions for Use (RUO)
Example Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
Product Brochure
Product description
