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Human sInterleukin-2 Receptor ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:IL-2R, Interleukin-2 receptor alpha, CD25 antigen
  • Species:Human
Cat. No. Size Price

RAF106R 96 wells (1 kit) $721,83
PubMed Product Details
Technical Data


Sandwich ELISA, HRP-labelled antibody


The Human sIL-2R ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of Human sIL-2R. The Human sIL-2R ELISA is for research use only. Not for diagnostic or therapeutic procedures.


Serum, Plasma, Cell culture supernatant

Sample Requirements

50 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.31–20 ng/ml

Limit of Detection

0.27 ng/ml

Intra-assay (Within-Run)

CV = 7.2%

Inter-assay (Run-to-Run)

CV = 9.8%

Spiking Recovery

75.0 %

Dilution Linearity



The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a Human IL-2R positive serum. There was no crossreactivity detected.



  • RUO
  • calibration range 0.31-20 ng/ml
  • limit of detection 0.27 ng/ml
  • intra-assay CV = 7.2%
  • inter-assay CV = 9.8%

Research topic

Cytokines and chemokines and related molecules, Oncology, COVID-19


Interleukin-2 (IL-2) has been described as a factor that promotes the growth and proliferation of Human T-cells thus occupying a pivotal role in the generation of the immune response. This proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific cell surface receptor following T lymphocyte activation. The receptor for IL-2 is composed of at least three distinct polypeptide subunits, the IL-2R, IL-2R, and the IL-2R chains giving rise to a 55-65 kD membrane bound protein. The genes encoding IL-2 and these three receptor subunits have been cloned and their complete primary structures have been deduced. Evidence has accumulated that suggests a critical role of IL-2R in IL-2 signal transduction. IL-2R coupled tyrosine kinases are believed to play a crucial role in that signal transduction.

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