Cell culture lysate
10 – 100 µl/well
Store the complete kit at room temperature until opened. Under these conditions, all components are stable until the expiration date (see label on the box). Once opened, follow the storage conditions on component labels.
2 - 200µg/ml or 0.25 - 20µg/ml
Limit of Detection
2ug/ml or 0.25ug/ml
The Sircol™ 2.0 Soluble Collagen kit is Biocolor's latest quantitative soluble collagen assay, optimised for both in-vivo & in-vitro samples, the kit offers increased collagen sensitivity & specificity compared to our original Sircol S1000 assay
Wavelengths between 530 – 560nm are suitable for analysis
96 measurements per kit in total
Allows a maximum of 41 samples to be run in duplicate alongside a standard curve
Suitable samples: Liquid extracts containing soluble collagen; prior salt/acid/acid-pepsin extraction may be necessary to release soluble collagen
In-vivo: tissues, cartilages, and fluids
In-vitro: 2D/3D culture extracellular matrices/conditioned media
Reactivity: Soluble collagens containing intact triple-helix structure
As supplied, Sircol 2.0 is calibrated for samples of *mammalian origin
Collagen is a major constituent of the extracellular matrix and the predominate protein found in animals, making up approximately 30% of all protein mass. Collagens are characterised by possessing at least one ‘domain’ of triple helix structure. At least 28 different types of collagens have been identified, with Type I collagen being the most prevalent form, particularly in ligaments, tendons, skin, and bone tissue. The biophysical properties of mature, ‘Insoluble collagen’ allow it to withstand high tensile forces as well as being resistant to stretch. These are essential properties that enable the locomotion of organisms. Collagen also exhibits key biochemical properties, being involved in cell growth, proliferation, and differentiation. This 'biochemical' form of collagen is typically recently synthesised and due to minimal crosslinking, can be readily solubilised via cold acid / enzymatic digestion. This is commonly referred to as ‘Soluble Collagen’. Aged collagen undergoes extensive cross-linking and therefore remains ‘insoluble’ under cold acid / enzymatic digestion.
The Sircol range of collagen assay kits have been designed for user-friendly quantification of soluble collagens (Sircol 2.0 kit) or insoluble (cross-linked) collagens (S2000 kit). Under assay conditions, Sircol Dye reagent is formulated to bind specifically to the (Gly-X-Y)n helical structure of soluble collagen.