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SircolTM 2.0 Soluble Collagen Assay (96 assays)

  • Regulatory status:RUO
  • Type:Colorimetric assay
  • Species:Human
Cat. No. Size Price

New SIRC2 96 assays (1 kit) $520
PubMed Product Details
Technical Data


Colorimetric assay


Cell culture lysate

Sample Requirements

10 – 100 µl/well


Store the complete kit at room temperature until opened. Under these conditions, all components are stable until the expiration date (see label on the box). Once opened, follow the storage conditions on component labels.

Calibration Range

2 - 200µg/ml or 0.25 - 20µg/ml

Limit of Detection

2ug/ml or 0.25ug/ml



The Sircol™ 2.0 Soluble Collagen kit is Biocolor's latest quantitative soluble collagen assay, optimised for both in-vivo & in-vitro samples, the kit offers increased collagen sensitivity & specificity compared to our original Sircol S1000 assay

Wavelengths between 530 – 560nm are suitable for analysis

96 measurements per kit in total

Allows a maximum of 41 samples to be run in duplicate alongside a standard curve

Suitable samples: Liquid extracts containing soluble collagen; prior salt/acid/acid-pepsin extraction may be necessary to release soluble collagen

In-vivo: tissues, cartilages, and fluids

In-vitro: 2D/3D culture extracellular matrices/conditioned media

Reactivity: Soluble collagens containing intact triple-helix structure

As supplied, Sircol 2.0 is calibrated for samples of *mammalian origin

Research topic

Extracellular matrix


Collagen is a major constituent of the extracellular matrix and the predominate protein found in animals, making up approximately 30% of all protein mass. Collagens are characterised by possessing at least one ‘domain’ of triple helix structure. At least 28 different types of collagens have been identified, with Type I collagen being the most prevalent form, particularly in ligaments, tendons, skin, and bone tissue. The biophysical properties of mature, ‘Insoluble collagen’ allow it to withstand high tensile forces as well as being resistant to stretch. These are essential properties that enable the locomotion of organisms. Collagen also exhibits key biochemical properties, being involved in cell growth, proliferation, and differentiation. This 'biochemical' form of collagen is typically recently synthesised and due to minimal crosslinking, can be readily solubilised via cold acid / enzymatic digestion. This is commonly referred to as ‘Soluble Collagen’. Aged collagen undergoes extensive cross-linking and therefore remains ‘insoluble’ under cold acid / enzymatic digestion. The Sircol range of collagen assay kits have been designed for user-friendly quantification of soluble collagens (Sircol 2.0 kit) or insoluble (cross-linked) collagens (S2000 kit). Under assay conditions, Sircol Dye reagent is formulated to bind specifically to the (Gly-X-Y)n helical structure of soluble collagen.

Summary References (1)

References to Collagen

  • Ricard-Blum S. The collagen family. Cold Spring Harb Perspect Biol. 2011 Jan 1;3(1):a004978. doi: 10.1101/cshperspect.a004978. PMID: 21421911; PMCID: PMC3003457. See more on PubMed
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