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Testosterone Free, ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Human
Cat. No. Size Price

RCD016R 96 wells (1 kit) $403,42
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen


For the direct quantitative determination of Free Testosterone by enzyme immunoassay in human serum.



Sample Requirements

25 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.1–60 pg/ml

Limit of Detection

0.018 pg/ml

Intra-assay (Within-Run)

CV < 7%

Inter-assay (Run-to-Run)

CV < 12%



  • RUO
  • calibration range 0.1-60 pg/ml
  • limit of detection 0.018 pg/ml
  • controls

Research topic

Steroid hormones


Testosterone is a C-19 steroid secreted from the testis and the adrenal cortex in men and from the adrenal cortex and ovaries in women. Testosterone is also produced by peripheral tissues from androstenedione, which is of little physiological significance in men, however in women about half of the circulating testosterone is derived from this origin. Testosterone measurements are used mainly for clinical evaluation of hypogonadism in males and hyperandrogenic states in females. Testosterone circulates in the blood bound to three proteins: sex hormone binding globulin (60-80%), albumin and cortisol binding globulin. Only about 1-2% of the total circulating testosterone remains unbound or free. Even though it is still under investigation, most researchers accept the free testosterone determination as a measure of the biologically active fraction. Free testosterone determinations are recommended to overcome the influences caused by variations in transport proteins on the total testosterone concentration.

Summary References (16)

References to Testosterone

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  • Ekins R. Hirsutism: free and bound testosterone. Ann Clin Biochem. 1990 Jan;27 ( Pt 1):91-4
  • Joshi UM, Shah HP, Sudhama SP. A sensitive and specific enzymeimmunoassay for serum testosterone. Steroids. 1979 Jul;34 (1):35-46
  • Manni A, Pardridge WM, Cefalu W, Nisula BC, Bardin CW, Santner SJ, Santen RJ. Bioavailability of albumin-bound testosterone. J Clin Endocrinol Metab. 1985 Oct;61 (4):705-10
  • Marcus GJ, Durnford R. A simple enzyme-linked immunosorbent assay for testosterone. Steroids. 1985 Dec;46 (6):975-86
  • Oka K, Hirano T, Noguchi M. Changes in the concentration of testosterone in serum during the menstrual cycle, as determined by liquid chromatography. Clin Chem. 1988 Mar;34 (3):557-60
  • Palatsi R, Hirvensalo E, Liukko P, Malmiharju T, Mattila L, Riihiluoma P, Ylostalo P. Serum total and unbound testosterone and sex hormone binding globulin (SHBG) in female acne patients treated with two different oral contraceptives. Acta Derm Venereol. 1984;64 (6):517-23
  • Pardridge WM, Demers LM. Bioavailable testosterone in salivary glands. Clin Chem. 1991 Feb;37 (2):139-40
  • Rassaie MJ, Kumari GL, Rao PN, Shrivastav TG, Pandey HP. Influence of different combinations of antibodies and penicillinase-labeled testosterone derivatives on sensitivity and specificity of immunoassays. Steroids. 1992 Mar;57 (3):112-8
  • Sheldon PE, Coppenger CJ. A rapid radioimmunoassay for serum testosterone. Steroids. 1977 Aug;30 (2):149-57
  • Singer F, Zumoff B. Subnormal serum testosterone levels in male internal medicine residents. Steroids. 1992 Feb;57 (2):86-9
  • Swinkels LM, Meulenberg PM, Ross HA, Benraad TJ. Salivary and plasma free testosterone and androstenedione levels in women using oral contraceptives containing desogestrel or levonorgestrel. Ann Clin Biochem. 1988 Jul;25 ( Pt 4):354-9
  • Swinkels LM, Ross HA, Benraad TJ. A symmetric dialysis method for the determination of free testosterone in human plasma. Clin Chim Acta. 1987 Jun 15;165 (2-3):341-9
  • Tateiki, K., et al. Enzymeimmunoassay of serum Testosterone. Steroids. 30:25, 1977;
  • Wilke TJ, Utley DJ. Total testosterone, free-androgen index, calculated free testosterone, and free testosterone by analog RIA compared in hirsute women and in otherwise-normal women with altered binding of sex-hormone-binding globulin. Clin Chem. 1987 Aug;33 (8):1372-5
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