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Manufactured by BioVendor

Mouse/Rat Testosterone ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA
  • Species:Mouse, Rat
Cat. No. Size Price

RTC001R 96 wells (1 kit) $570,15
PubMed Product Details
Technical Data


Competitive ELISA


The BioVendor Testosterone rat/mouse ELISA is a competitive immunoassay for the measurement of testosterone in rat and mouse serum or plasma (EDTA). For research use only. Not for use in diagnostic procedures.


Serum, Plasma-EDTA

Sample Requirements

10 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.1–25 ng/ml

Limit of Detection

0.024 ng/ml



  • RUO
  • calibration range 0.1-25 ng/ml
  • limit od detection 0.024 ng/ml

Research topic

Steroid hormones, Animal studies


Testosterone is a steroid hormone from the androgen group synthesized by the Leydig cells in the testes in males, the ovaries in females, and adrenal glands in both sexes. It exerts a wide-ranging influence on sexual behaviour, muscle mass and strength, energy, cardiovascular health, and bone integrity. Testosterone biosynthesis coincides with the spermatogenesis and fetal Leydig cell differentiation in the male rat. Several in vivo models including hormone suppression, hormone restoration and hypophysectomy were established for the study of the hormonal regulation of spermatogenesis by testosterone (1-3). In the Brown Norway rat, serum testosterone levels decrease with aging, accompanied by increases in serum FSH. The capacity of Leydig cells to produce testosterone is higher in young than in old rats (4). Testosterone secreted during late gestational and neonatal periods causes significant brain sexual dimorphism in the rat. This results in both sex-specific behaviour and endocrinology in adults (5). Analyses concerning the regulation of synthesis reveal that testosterone is able to regulate its own synthesis and indicate that this autoregulation is the result of rapid, specific inhibition by testosterone of 17 alpha-hydroxylase activity (6).

Product References (3)


  • Barkallah M, Slima AB, Elleuch F, Fendri I, Pichon C, Abdelkafi S, Baril P. Protective Role of Spirulina platensis Against Bifenthrin-Induced Reprotoxicity in Adult Male Mice by Reversing Expression of Altered Histological, Biochemical, and Molecular Markers Including MicroRNAs. Biomolecules. 2020 May 12;10(5):753. doi: 10.3390/biom10050753. PubMed PMID: 32408700. PubMed CentralPMCID: PMC7277961. See more on PubMed
  • Skovmand A, Erdely A, Antonini JM, Nurkiewicz TR, Shoeb M, Eye T, Kodali V, Loeschner K, Vidmar J, Agerholm JS, Goericke-Pesch S, Vogel U, Hougaard KS. Inhalation of welding fumes reduced sperm counts and high fat diet reduced testosterone levels; differential effects in Sprague Dawley and Brown Norway rats. Part Fibre Toxicol. 2020 Jan 10;17(1):2. doi: 10.1186/s12989-019-0334-0. PubMed PMID: 31924220. PubMed CentralPMCID: PMC6954601. See more on PubMed
  • Mishra JS, Blesson CS, Kumar S. Testosterone Decreases Placental Mitochondrial Content and Cellular Bioenergetics. Biology (Basel). 2020 Jul 20;9(7):176. doi: 10.3390/biology9070176. PubMed PMID: 32698476. PubMed CentralPMCID: PMC7407169. See more on PubMed
Summary References (16)

References to Testosterone

  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Dechaud H, Lejeune H, Garoscio-Cholet M, Mallein R, Pugeat M. Radioimmunoassay of testosterone not bound to sex-steroid-binding protein in plasma. Clin Chem. 1989 Aug;35 (8):1609-14
  • Ekins R. Hirsutism: free and bound testosterone. Ann Clin Biochem. 1990 Jan;27 ( Pt 1):91-4
  • Joshi UM, Shah HP, Sudhama SP. A sensitive and specific enzymeimmunoassay for serum testosterone. Steroids. 1979 Jul;34 (1):35-46
  • Manni A, Pardridge WM, Cefalu W, Nisula BC, Bardin CW, Santner SJ, Santen RJ. Bioavailability of albumin-bound testosterone. J Clin Endocrinol Metab. 1985 Oct;61 (4):705-10
  • Marcus GJ, Durnford R. A simple enzyme-linked immunosorbent assay for testosterone. Steroids. 1985 Dec;46 (6):975-86
  • Oka K, Hirano T, Noguchi M. Changes in the concentration of testosterone in serum during the menstrual cycle, as determined by liquid chromatography. Clin Chem. 1988 Mar;34 (3):557-60
  • Palatsi R, Hirvensalo E, Liukko P, Malmiharju T, Mattila L, Riihiluoma P, Ylostalo P. Serum total and unbound testosterone and sex hormone binding globulin (SHBG) in female acne patients treated with two different oral contraceptives. Acta Derm Venereol. 1984;64 (6):517-23
  • Pardridge WM, Demers LM. Bioavailable testosterone in salivary glands. Clin Chem. 1991 Feb;37 (2):139-40
  • Rassaie MJ, Kumari GL, Rao PN, Shrivastav TG, Pandey HP. Influence of different combinations of antibodies and penicillinase-labeled testosterone derivatives on sensitivity and specificity of immunoassays. Steroids. 1992 Mar;57 (3):112-8
  • Sheldon PE, Coppenger CJ. A rapid radioimmunoassay for serum testosterone. Steroids. 1977 Aug;30 (2):149-57
  • Singer F, Zumoff B. Subnormal serum testosterone levels in male internal medicine residents. Steroids. 1992 Feb;57 (2):86-9
  • Swinkels LM, Meulenberg PM, Ross HA, Benraad TJ. Salivary and plasma free testosterone and androstenedione levels in women using oral contraceptives containing desogestrel or levonorgestrel. Ann Clin Biochem. 1988 Jul;25 ( Pt 4):354-9
  • Swinkels LM, Ross HA, Benraad TJ. A symmetric dialysis method for the determination of free testosterone in human plasma. Clin Chim Acta. 1987 Jun 15;165 (2-3):341-9
  • Tateiki, K., et al. Enzymeimmunoassay of serum Testosterone. Steroids. 30:25, 1977;
  • Wilke TJ, Utley DJ. Total testosterone, free-androgen index, calculated free testosterone, and free testosterone by analog RIA compared in hirsute women and in otherwise-normal women with altered binding of sex-hormone-binding globulin. Clin Chem. 1987 Aug;33 (8):1372-5
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