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Urocortin 2 Mouse ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
  • Other names:Ucn 2, Stresscopin-Related Peptide
  • Species:Mouse
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RSCYK190R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antibody

Applications

Serum, Plasma

Sample Requirements

20 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.82–200 ng/ml

Intra-assay (Within-Run)

Mouse plasma: CV = 2.5-5.3%
Mouse serum: CV = 6.7-9.0%

Inter-assay (Run-to-Run)

Mouse plasma: CV = 4.7-8.3%
Mouse serum: CV = 6.4-11.1%

Spiking Recovery

Mouse plasma: 107.8%
Mouse serum: 121.7%

Summary

Research topic

Animal studies

Summary

Urocortin 2 (Ucn 2), also known as stresscopin-related peptide, is a novel predicted neuropeptide related to
corticotropin-releasing factor (CRF). The peptide consisting of 38 amino acid residues was first demonstrated to be expressed centrally and to bind selectively to type 2 CRF receptor (CRFR2). In the rodent, Ucn 2 transcripts were shown to be expressed in the discrete regions of the central nervous system including stress-related cell groups in the hypothalamus and brainstem. More recently, the expression of Ucn 2 transcripts was detected in the olfactory bulb, pituitary, cortex, hypothalamus, and spinal cord. Ucn 2 mRNA was also found to be expressed widely in a variety of peripheral tissues, most highly in the skin and skeletal muscle tissues. Ucn 2-like immunoreactivity was detected by RIA in acid extracts of mouse brain, muscle, and skin. Immunohistochemically Ucn 2 was found in both skin epidermis and adnexal structures and in the skeletal muscle myocytes. Ucn 2 gene transcription was stimulated in the hypothalamus and brainstem by glucocorticoid administration to the mouse and inhibited by removal of glucocorticoids by adrenalectomy, suggesting a putative link between the CRFR1 and CRFR2 pathways. On the other hand, in the rat a stressor-specific regulation of Ucn 2 mRNA expression in the hypothalamic paraventricular nucleus was demonstrated, which raised the possibility of a modulary role of Ucn 2 mRNA in stress-induced alteration of anterior and posterior pituitary function, depending on the type of stress. Administration of dexamethasone to the
mouse resulted in a decrease of Ucn 2 mRNA levels in the back skin region. Adrenalectomy signifcantly increased Ucn 2 mRNA levels in the skin, and the levels were reduced back to normal levels after corticoid replacement. CRFR2 is found in cardiomyocytes and in endothelial and smooth muscle cells of the systemic vasculature. Ucn 2 is expressed in the mouse cardiomyocytes. In the mouse, Ucn 2 treatment augmented heart rate, exhibited potent inotropic and lusitropic actions on the left ventricle, and induced a downward shift of the diastolic pressure-volume relation. Ucn 2 also reduced systemic arterial pressure, associated with a lowering of systemic arterial elastance and systemic vascular resistance. The effects of Ucn 2 were specific to CRFR2 function and independent of beta-adrenergic receptors. These experiments demonstrated the potent cardiovascular physiologic actions of Ucn 2 in the both wild-type and cardiomyopathic mice and support a potential beneficial use of Ucn 2 in congestive heart failure treatment. The use of Ucn 2 was also proposed to treat ischemic heart disease because of its potent cardioprotective effect in the mouse heart and its minimal impact on the hypothalamic stress axis. Administration of Ucn 2 to the mouse prevented the loss of skeletal muscle mass resulting from disuse due to casting, corticosteroid treatment, and nerve damage. In addition, Ucn 2 treatment prevented the loss of skeletal muscle force and myocyte cross-sectional area that accompanied muscle mass losses resulting from disuse due to casting. In normal muscles of the mouse, Ucn 2 increased skeletal muscle mass and force. It was thus proposed that Ucn 2 might find utility in the treatment of skeletal muscle wasting diseases including age-related muscle loss or sarcopenia. Mouse urocortin 2 (Ucn 2) is a new peptide predicted from mouse cDNA sequence and its physiologic and pathophysiologic significance has not yet been fully elucidated. However, the experimental data presented to date provided evidence for the important physiologic roles of Ucn 2 and urge the necessity of further investigation of the peptide from various points of view.

Product References (7)

References

  • Bale TL, Hoshijima M, Gu Y, Dalton N, Anderson KR, Lee KF, Rivier J, Chien KR,Vale WW, Peterson KL. The cardiovascular physiologic actions of urocortin II:acute effects in murine heart failure. Proc Natl Acad Sci U S A. 2004 Mar9;101(10):3697-702. Epub 2004 Feb 27. PubMed PMID: 14990799; PubMed CentralPMCID: PMC373525. See more on PubMed
  • Brar BK, Jonassen AK, Egorina EM, Chen A, Negro A, Perrin MH, Mjøs OD,Latchman DS, Lee KF, Vale W. Urocortin-II and urocortin-III are cardioprotective against ischemia reperfusion injury: an essential endogenous cardioprotectiverole for corticotropin releasing factor receptor type 2 in the murine heart.Endocrinology. 2004 Jan;145(1):24-35; discussion 21-3. Epub 2003 Sep 11. PubMedPMID: 12970163. See more on PubMed
  • Hinkle RT, Donnelly E, Cody DB, Bauer MB, Isfort RJ. Urocortin II treatmentreduces skeletal muscle mass and function loss during atrophy and increasesnonatrophying skeletal muscle mass and function. Endocrinology. 2003Nov;144(11):4939-46. Epub 2003 Jul 31. PubMed PMID: 12960070. See more on PubMed
  • Chen A, Vaughan J, Vale WW. Glucocorticoids regulate the expression of themouse urocortin II gene: a putative connection between thecorticotropin-releasing factor receptor pathways. Mol Endocrinol. 2003Aug;17(8):1622-39. Epub 2003 May 22. PubMed PMID: 12764078. See more on PubMed
  • Chen A, Blount A, Vaughan J, Brar B, Vale W. Urocortin II gene is highlyexpressed in mouse skin and skeletal muscle tissues: localization, basalexpression in corticotropin-releasing factor receptor (CRFR) 1- and CRFR2-nullmice, and regulation by glucocorticoids. Endocrinology. 2004 May;145(5):2445-57. Epub 2004 Jan 21. PubMed PMID: 14736736. See more on PubMed
  • Reyes TM, Lewis K, Perrin MH, Kunitake KS, Vaughan J, Arias CA, Hogenesch JB, Gulyas J, Rivier J, Vale WW, Sawchenko PE. Urocortin II: a member of thecorticotropin-releasing factor (CRF) neuropeptide family that is selectivelybound by type 2 CRF receptors. Proc Natl Acad Sci U S A. 2001 Feb27;98(5):2843-8. PubMed PMID: 11226328; PubMed Central PMCID: PMC30227. See more on PubMed
  • Tanaka Y, Makino S, Noguchi T, Tamura K, Kaneda T, Hashimoto K. Effect ofstress and adrenalectomy on urocortin II mRNA expression in the hypothalamicparaventricular nucleus of the rat. Neuroendocrinology. 2003 Jul;78(1):1-11.PubMed PMID: 12869794. See more on PubMed
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