Native protein isolated from human urine, 590 AA, 64,265 kDa (calculated without glycosylation). Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
Determined by GFC analysis: >95%
12% SDS-PAGE separation of Human Uromodulin
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 2.5μg / lane
3. non-reduced and non-heated sample, 2.5μg / lane
< 0.1 EU/μg
Filtered (0.4 μm) and lyophilized from 0.5 mg/mL solution in water for injection.
Add water for injection to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Western blotting, ELISA
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
GFC to determine purity of the protein.
LAL to determine quantity of endotoxin.
All donors of urine samples used for protein preparation were tested and found negative for HBsAg, anti-HCV, HIV Ag/Ab, and syphilis. Since no test can absolutely assure the absence of all infectious agents, this product should be handled as a potential biohazard. This product is intended for research use only.