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Club Cell Protein (CC16) Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:CC10, CC16, uteroglobin, urinary protein 1, Clara Cell Secretory Protein, Casein kinase 1, CK1
  • Species:Human
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Cat. No. Size Price


RD191022200 96 wells (1 kit)
PubMed Product Details
Technical Data

Product has been modified

Clara Cell Protein Human ELISA has been renamed to Club Cell Protein (CC16) Human ELISA. This change respects a name-change policy of the major respiratory journals (including the journals of the American Thoracic Society, the European Respiratory Society and the American College of Chest Physicians) that went into effect beginning January 1, 2013.

Should you have any question, please contact the Technical Support Department.

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

5 µl/well

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1.57–50 ng/ml

Limit of Detection

46 pg/ml

Intra-assay (Within-Run)

n = 8; CV = 3.4%

Inter-assay (Run-to-Run)

n = 4; CV = 4.7%

Spiking Recovery

96,00%

Dilutation Linearity

103,60%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • monkey Yes
  • mouse Yes
  • human Yes
Summary

Features

  • European Union: for in vitro diagnostic use
  • Rest of the world: for research use only!
  • The total assay time is less than 4 hours
  • The kit measures total Club cell protein in serum and plasma (EDTA, citrate, heparin)
  • Assay format is 96 wells
  • Quality Controls are human serum based
  • Standard is recombinant protein (E. coli) based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Clinical use and areas of investigation:

  • Pneumonia and bronchopneumonia
  • Chronic obstructive pulmonary disease, sarcoidosis, pulmonary fibrosis
  • Acute lung injury
  • Asthma and allergic rhinitis
  • Lung cancer
  • IgA-nephropathy

Research topic

Immune Response, Infection and Inflammation, Pulmonary diseases

Summary

Human Club cell protein (CC16, CC10, uteroglobin, urinary protein 1 or Clara cell secretory
protein) is a member of the secretoglobin family of proteins and is a secreted product of nonciliated
bronchiolar Club cells. Its function remains to be fully elucidated but there is
convincing data suggesting its role as an immune-modulating and anti-inflammatory agent.
Club cell protein inhibits phospholipase A2 activity as well as interferon gamma signaling and
Th1 vs. Th2 lymphocyte regulation.

Club cell protein concentrations have been determined in serum, plasma and bronchoalveolar
lavage fluid in numerous studies since 1994. In serum, its increase is associated with age and
asbestos, nitrogen chloride and ozone exposure. Higher levels of CC16 were demonstrated in
patients with sarcoidosis, pulmonary fibrosis and high PEEP ventilation. Decreased serum
CC16 levels are found after pulmonary resection in smokers and in subjects with chronic
obstructive pulmonary disease, asthma or silica exposure.

Decreased CC16 concentrations were also found in the amniotic fluid of fetuses suffering from
pulmonary hypoplasia caused by various mechanisms (diaphragmatic hernia, diabetic
fetopathy, Turner and Down syndrome). In pleural effusions, the CC16 concentration appears
to be associated with its diffusion from the lung as evidenced by high CC16 levels in cardiac
pleural congestion.
Based on the above reports Club cell protein might be perspective useful diagnostic marker of
pulmonary diseases and injuries.

References to Product

References

  • Alexis NE, Lay JC, Haczku A, Gong H, Linn W, Hazucha MJ, Harris B, Tal-Singer R, Peden DB. Fluticasone propionate protects against ozone-induced airway inflammation and modified immune cell activation markers in healthy volunteers. Environ Health Perspect. 2008 Jun;116 (6):799-805
  • Andersson L, Lundberg PA, Barregard L. Methodological aspects on measurement of Clara cell protein in urine as a biomarker for airway toxicity, compared with serum levels. J Appl Toxicol . Dec 22;27(1):60-66 (2006)
  • Aul R, Armstrong J, Duvoix A, Lomas D, Hayes B, Miller BE, Jagger C, Singh D. Inhaled LPS challenges in smokers: a study of pulmonary and systemic effects. Br J Clin Pharmacol. 2012 Dec;74 (6):1023-32
  • Benson M, Fransson M, Martinsson T, Naluai AT, Uddman R, Cardell LO. Inverse relation between nasal fluid Clara Cell Protein 16 levels and symptoms and signs of rhinitis in allergen-challenged patients with intermittent allergic rhinitis. Allergy . Feb;62(2):178-83 (2007)
  • Benson M, Jansson L, Adner M, Luts A, Uddman R, Cardell LO. Gene profiling reveals decreased expression of uteroglobin and other anti-inflammatory genes in nasal fluid cells from patients with intermittent allergic rhinitis. Clin Exp Allergy . Apr;35(4):473-8 (2005)
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  • Chen Y, Vasquez MM, Zhu L, Lizarraga RE, Krutzsch M, Einspahr J, Alberts DS, Di PY, Martinez FD, Guerra S. Effects of Retinoids on Augmentation of Club Cell Secretory Protein. Am J Respir Crit Care Med. 2017 Feb 23. doi: 10.1164/rccm.201608-1611LE
  • Chimenti L, Morici G, Paterno A, Bonanno A, Vultaggio M, Bellia V, Bonsignore MR. Environmental conditions, air pollutants, and airway cells in runners: a longitudinal field study. J Sports Sci. 2009 Jul;27 (9):925-35
  • Coppens JT, Van Winkle LS, Pinkerton K, Plopper CG. Distribution of Clara cell secretory protein expression in the tracheobronchial airways of rhesus monkeys. Am J Physiol Lung Cell Mol Phy. 2007 May;292 (5):L1155-62
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  • Gaber F, Daham K, Higashi A, Higashi N, Gulich A, Delin I, James A, Skedinger M, Gyllfors P, Nord M, Dahlen SE, Kumlin M, Dahlen B. Increased levels of cysteinyl-leukotrienes in saliva, induced sputum, urine and blood from patients with aspirin-intolerant asthma. Thorax. 2008 Dec;63 (12):1076-82
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  • Guzmán-Bárcenas J, Calderón-Moore A, Baptista-González H, Irles C. Clara Cell Protein Expression in Mechanically Ventilated Term and Preterm Infants with Respiratory Distress Syndrome and at Risk of Bronchopulmonary Dysplasia: A Pilot Study. Can Respir J. 2017;2017:8074678. doi: 0.1155/2017/8074678
  • Harvey BG, Heguy A, Leopold PL, Carolan BJ, Ferris B, Crystal RG. Modification of gene expression of the small airway epithelium in response to cigarette smoking. J Mol Med . Jan;85(1):39-53 (2007)
  • Heldal KK, Barregard L, Larsson P, Ellingsen DG. Pneumoproteins in sewage workers exposed to sewage dust. Int Arch Occup Environ Health. 2013 Jan;86 (1):65-70
  • Ho JC, Au WY, Han L, Kwong YL, Ip MS. Effect of therapeutic arsenic exposure on pulmonary function. Respir Med. 2013 Sep;107 (9):1423-30
  • Holz O, Tan L, Schaumann F, Muller M, Scholl D, Hidi R, McLeod A, Krug N, Hohlfeld JM. Inter- and intrasubject variability of the inflammatory response to segmental endotoxin challenge in healthy volunteers. Pulm Pharmacol Ther. 2015 Dec;35:50-9
  • Huttmann EM, Greulich T, Hattesohl A, Schmid S, Noeske S, Herr C, John G, Jorres RA, Muller B, Vogelmeier C, Koczulla AR. Comparison of two devices and two breathing patterns for exhaled breath condensate sampling. PLoS One. 2011;6 (11):e27467
  • Ilic N, Mihailovic N. Serum Clara cell protein and atopic phenotype in children up to 2 years of age. J Clin Lab Anal. 2017 Feb 1. doi: 10.1002/jcla.22151
  • Karottki DG, Spilak M, Frederiksen M, Jovanovic Andersen Z, Madsen AM, Ketzel M, Massling A, Gunnarsen L, Moller P, Loft S. Indoor and outdoor exposure to ultrafine, fine and microbiologically derived particulate matter related to cardiovascular and respiratory effects in a panel of elderly urban citizens. Int J Environ Res Public Healt. 2015 Feb;12 (2):1667-86
  • Kippelen P, Tufvesson E, Ali L, Bjermer L, Anderson SD. Urinary CC16 after challenge with dry air hyperpnoea and mannitol in recreational summer athletes. Respiratory Medicine. 2 October 2013;
  • Knabe L, Varilh J, Bergougnoux A, Gamez AS, Bonini J, Pommier A, Petit A, Molinari N, Vachier I, Taulan-Cadars M, Bourdin A. CCSP G38A polymorphism-environment interactions regulate CCSP levels differentially in COPD. Am J Physiol Lung Cell Mol Phy. 2016 Aug 5;:ajplung.00280.2016
  • Kokuho N, Ishii T, Kamio K, Hayashi H, Kurahara M, Hattori K, Motegi T, Azuma A, Gemma A, Kida K. Diagnostic Values For Club Cell Secretory Protein (CC16) in Serum of Patients of Combined Pulmonary Fibrosis and Emphysema. COPD. 2015 Aug;12 (4):347-54
  • Kropski JA, Fremont RD, Calfee CS, Ware LB. Clara cell protein (CC16), a marker of lung epithelial injury, is decreased in plasma and pulmonary edema fluid from patients with acute lung injury. Chest. 2009 Jun;135 (6):1440-7
  • Kucejko W, Chyczewska E, Naumnik W, Ossolinska M. Concentration of surfactant protein D, Clara cell protein CC-16 and IL-10 in bronchoalveolar lavage (BAL) in patients with sarcoidosis, hypersensivity pneumonitis and idiopathic pulmonary fibrosis. Folia Histochem Cytobiol. 2009;47 (2):225-30
  • Liu Z, Lu X, Zhang XH, Bochner BS, Long XB, Zhang F, Wang H, Cui YH. Clara cell 10-kDa protein expression in chronic rhinosinusitis and its cytokine-driven regulation in sinonasal mucosa. Allergy. 2009 Jan;64 (1):149-57
  • Lomas DA, Silverman EK, Edwards LD, Miller BE, Coxson HO, Tal-Singer R. Evaluation of serum CC-16 as a biomarker for COPD in the ECLIPSE cohort. Thorax. 2008 Dec;63 (12):1058-63
  • Madsen C, Durand KL, Nafstad P, Schwarze PE, Ronningen KS, Haheim LL. Associations between environmental exposures and serum concentrations of Clara cell protein among elderly men in Oslo, Norway. Environ Res. 2008 Nov;108 (3):354-60
  • Martin AC, Laing IA, Khoo SK, Zhang G, Rueter K, Teoh L, Taheri S, Hayden CM, Geelhoed GC, Goldblatt J, LeSouef PN. Acute asthma in children: Relationships among CD14 and CC16 genotypes, plasma levels, and severity. Am J Respir Crit Care Med . Mar 15 173(6):617-22 (2006)
  • Mattsson J, Remberger M, Andersson O, Sundberg B, Nord M. Decreased serum levels of clara cell secretory protein (CC16) are associated with bronchiolitis obliterans and may permit early diagnosis in patients after allogeneic stem-cell transplantation. Transplantation. 2005 May 27;79 (10):1411-6
  • Mickleborough TD, Vaughn CL, Shei RJ, Davis EM, Wilhite DP. Marine lipid fraction PCSO-524 (lyprinol/omega XL) of the New Zealand green lipped mussel attenuates hyperpnea-induced bronchoconstriction in asthma. Respir Med. 2013 Aug;107 (8):1152-63
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  • Romberg K, Bjermer L, Tufvesson E. Exercise but not mannitol provocation increases urinary Clara cell protein (CC16) in elite swimmers. Respir Med. 2010 Aug 7;
  • Sarafidis K, Stathopoulou T, Diamanti E, Soubasi V, Agakidis C, Balaska A, Drossou V. Clara cell secretory protein (CC16) as a peripheral blood biomarker of lung injury in ventilated preterm neonates. Eur J Pediatr. 2008 Nov;167 (11):1297-303
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  • Tufvesson E, Svensson H, Ankerst J, Bjermer L. Increase of club cell (Clara) protein (CC16) in plasma and urine after exercise challenge in asthmatics and healthy controls, and correlations to exhaled breath temperature and exhaled nitric oxide. Respir Med. 2013 Sep 6;
  • Ulvestad B, Randem BG, Andersson L, Ellingsen DG, Barregard L. Clara cell protein as a biomarker for lung epithelial injury in asphalt workers. J Occup Environ Med. 2007 Oct;49 (10):1073-8
  • Um SJ, Lam S, Coxson H, Man SF, Sin DD. Budesonide/formoterol enhances the expression of pro Surfactant Protein-B in lungs of COPD patients. PLoS One. 2013;8 (12):e83881
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  • Wutzler S, Lehnert T, Laurer H, Lehnert M, Becker M, Henrich D, Vogl T, Marzi I. Circulating levels of Clara cell protein 16 but not surfactant protein D identify and quantify lung damage in patients with multiple injuries. J Trauma. 2011 Aug;71 (2):E31-6
  • Yang KD, Ou CY, Chang JC, Chen RF, Liu CA, Liang HM, Hsu TY, Chen LC, Huang SK. Infant frequent wheezing correlated to Clara cell protein 10 (CC10) polymorphism and concentration, but not allergy sensitization, in a perinatal cohort study. J Allergy Clin Immunol. 2007 Oct;120 (4):842-8
References to Summary

References to Club Cell Protein

  • Deraz T, Kamel TB, El-Mogy MI, Moustafa EH. Serum and nasal lavage fluid Clara cell protein decreases in children with allergic rhinitis. Int J Pediatr Otorhinolaryngol, 76(9):1241–4 (2012)
  • Wuetzler S, Backhaus L, Henrich D, Geiger E: Clara cell protein16: A biomarker for detecting secondary respiratory complications in patients with multiple injuries. J Trauma Acute Care Surgery 73(4): 838–842 (2012)
  • Chowdhury B, Zhang Z, Mukherjee AB. Uteroglobin interacts with the heparin-binding site of fibronectin and prevents fibronectin-IgA complex formation found in IgA-nephropathy. FEBS Lett. 82(5):611–5 (2008)
  • Braido F, Riccio AM, Guerra L, Gamalero C, Zolezzi A, Tarantini F, De Giovanni B, Folli C, Descalzi D, Canonica GW. Clara cell 16 protein in COPD sputum: a marker of small airways damage? Respir Med. 101(10):2119–24 (2007)
  • Shijubo N., Kawabata I., Sato N., Itoh Y.: Clinical Aspects of Clara Cell 10-kDa Protein/ Uteroglobin (Secretoglobin 1A1), Current Pharmaceutical Design, 9, 1139–1149, (2003)
  • Nord M., Schubert K., Cassel T., Andersson O., Riise G.: Decreased serum and bronchoalveolar lavage levels of Clara cell secretory protein (CC16) is associated with bronchiolitis obliterans syndrome and airway neutrophilia in lung transplant recipients. Transplantation, 73, 1264–1269, (2002)
  • Petrek M., Hermans C., Kolek V., Fialova J., Bernard A.: Clara cell protein (CC16) in serum and bronchoalveolar lavage fluid of subjects exposed to asbestos. Biomarkers, 7(1), 58–67, (2002)
  • Hermans C., Petrek M., Kolek V., Weynand B., Pieters T., Lambert M., Bernard A.: Serum Clara cell protein (CC16), a marker of the integrity of the air-blood barrier in sarcoidosis. Eur Respir J, 18(3), 507–514 (2001)
  • Bernard A., Roels H., Lauwerys R., Witters R., Gielens C., Soumillion A. et al.: Human urinary protein 1: Evidence for identity with the Clara cell protein and occurrence in respiratory tract and urogenital secretions. Clin Chim Acta, 207, 239–249, (1992)
  • Bernard A., Lauwerys R., Noel A., Vandeleene B., Lambert A.: Urine protein 1: a sexdependent marker of tubular or glomerular dysfunction. Clin Chem, 35, 2141–2142, (1989)
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