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Manufactured by BioVendor

Club Cell Protein (CC16) Human, Rabbit Polyclonal Antibody

  • Regulatory status:RUO
  • Type:Polyclonal Antibody
  • Other names:CC10, CC16, uteroglobin, urinary protein 1, Clara Cell Secretory Protein, Casein kinase 1, CK1
  • Species:Human
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Cat. No. Size Price


RD181022220-01 0.1 mg
PubMed Product Details
Technical Data

Product has been modified

Clara Cell Protein Human ELISA has been renamed to Club Cell Protein (CC16) Human ELISA. This change respects a name-change policy of the major respiratory journals (including the journals of the American Thoracic Society, the European Respiratory Society and the American College of Chest Physicians) that went into effect beginning January 1, 2013.

Should you have any question, please contact the Technical Support Department.

Type

Polyclonal Antibody

Applications

Western blotting, ELISA, Immunohistochemistry

Antibodies Applications

Source of Antigen

Human urine

Hosts

Rabbit

Preparation

The antibody was raised in rabbits by immunization with the Human Club Cell Protein.

Species Reactivity

Human

Purification Method

Immunoaffinity chromatography on a column with immobilized recombinant Human Club Cell Protein (RD172022100, Biovendor).

Antibody Content

0.1 mg (determined by BCA method, BSA was used as a standard)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody SDS PAGE – to determine purity of the antibody

Summary

Research topic

Immune Response, Infection and Inflammation, Pulmonary diseases

Summary

Human Club Cell Protein (CC16, CC10 and also called uteroglobin, urinary protein 1 or Clara Cell Secretory Protein) belongs to the family of secretoglobins and is a secreted protein product of non-ciliated bronchiolar Club cells. Its function remains to be elucidated but there is convincing data suggesting its phospholipase A2 inhibitory activity as well as a number of other immunomodulatory features including inhibition of interferon gamma signaling and Th1 vs. Th2 lymphocyte regulation. It was proposed as a potential peripheral marker of respiratory epithelial injury and bronchial dysfunction. In serum, its increase is associated with age, asbestos, nitrogen chloride and ozone exposure, sarcoidosis and high PEEP ventilation. Decreased serum CC16 levels are found after pulmonary resection, in silica-exposed workers, smokers and in asthma. Decreased CC16 concentrations were also found in the amniotic fluid of fetuses suffering from pulmonary hypoplasia caused by various mechanisms (diaphragmatic hernia, diabetic fetopathy, Turner and Down syndrome).

Product References (8)

References

  • Coppens JT, Van Winkle LS, Pinkerton K, Plopper CG. Distribution of Clara cellsecretory protein expression in the tracheobronchial airways of rhesus monkeys.Am J Physiol Lung Cell Mol Physiol. 2007 May;292(5):L1155-62. Epub 2007 Jan 19.PubMed PMID: 17237148. See more on PubMed
  • Fakhiri J, Schneider MA, Puschhof J, Stanifer M, Schildgen V, Holderbach S,Voss Y, El Andari J, Schildgen O, Boulant S, Meister M, Clevers H, Yan Z, Qiu J, Grimm D. Novel Chimeric Gene Therapy Vectors Based on Adeno-Associated Virus and Four Different Mammalian Bocaviruses. Mol Ther Methods Clin Dev. 2019 Jan18;12:202-222. doi: 10.1016/j.omtm.2019.01.003. eCollection 2019 Mar 15. PubMedPMID: 30766894; PubMed Central PMCID: PMC6360332. See more on PubMed
  • Greeley MA, Van Winkle LS, Edwards PC, Plopper CG. Airway trefoil factorexpression during naphthalene injury and repair. Toxicol Sci. 2010Feb;113(2):453-67. doi: 10.1093/toxsci/kfp268. Epub 2009 Oct 30. PubMed PMID:19880587; PubMed Central PMCID: PMC2807036. See more on PubMed
  • Hüttmann EM, Greulich T, Hattesohl A, Schmid S, Noeske S, Herr C, John G,Jörres RA, Müller B, Vogelmeier C, Koczulla AR. Comparison of two devices and twobreathing patterns for exhaled breath condensate sampling. PLoS One.2011;6(11):e27467. doi: 10.1371/journal.pone.0027467. Epub 2011 Nov 7. Erratumin: PLoS One. 2016;11(3):e0152620. PubMed PMID: 22087323; PubMed Central PMCID:PMC3210176. See more on PubMed
  • Rau T, Dimmler A, Häfner M, Brabletz T, Kirchner T, Faller G. Aberrantexpression of TTF-1 and forkhead factor HFH-4 in atrophic gastritis and ciliated metaplasia suggests gastric broncho-pulmonary transdetermination. J Pathol. 2005 Aug;206(4):383-7. PubMed PMID: 15912575. See more on PubMed
  • Williams KM, Franzi LM, Last JA. Cell-specific oxidative stress andcytotoxicity after wildfire coarse particulate matter instillation into mouselung. Toxicol Appl Pharmacol. 2013 Jan 1;266(1):48-55. doi:10.1016/j.taap.2012.10.017. Epub 2012 Nov 7. PubMed PMID: 23142465; PubMedCentral PMCID: PMC3546532. See more on PubMed
  • Zhang H, Yang J, Walters MS, Staudt MR, Strulovici-Barel Y, Salit J, Mezey JG,Leopold PL, Crystal RG. Mandatory role of HMGA1 in human airway epithelial normaldifferentiation and post-injury regeneration. Oncotarget. 2018 Feb16;9(18):14324-14337. doi: 10.18632/oncotarget.24511. eCollection 2018 Mar 6.PubMed PMID: 29581847; PubMed Central PMCID: PMC5865673. See more on PubMed
  • Gomi K, Tang Y, Arbelaez V, Crystal RG, Walters MS. Endothelial Cell Mediated Promotion of Ciliated Cell Differentiation of Human Airway Basal Cells viaInsulin and Insulin-Like Growth Factor 1 Receptor Mediated Signaling. Stem CellRev. 2017 Apr;13(2):309-317. doi: 10.1007/s12015-016-9707-z. PubMed PMID:28050756; PubMed Central PMCID: PMC5584604. See more on PubMed
Summary References (10)

References to Club Cell Protein

  • Deraz T, Kamel TB, El-Mogy MI, Moustafa EH. Serum and nasal lavage fluid Clara cell protein decreases in children with allergic rhinitis. Int J Pediatr Otorhinolaryngol, 76(9):1241–4 (2012)
  • Wuetzler S, Backhaus L, Henrich D, Geiger E: Clara cell protein16: A biomarker for detecting secondary respiratory complications in patients with multiple injuries. J Trauma Acute Care Surgery 73(4): 838–842 (2012)
  • Chowdhury B, Zhang Z, Mukherjee AB. Uteroglobin interacts with the heparin-binding site of fibronectin and prevents fibronectin-IgA complex formation found in IgA-nephropathy. FEBS Lett. 82(5):611–5 (2008)
  • Braido F, Riccio AM, Guerra L, Gamalero C, Zolezzi A, Tarantini F, De Giovanni B, Folli C, Descalzi D, Canonica GW. Clara cell 16 protein in COPD sputum: a marker of small airways damage? Respir Med. 101(10):2119–24 (2007)
  • Shijubo N., Kawabata I., Sato N., Itoh Y.: Clinical Aspects of Clara Cell 10-kDa Protein/ Uteroglobin (Secretoglobin 1A1), Current Pharmaceutical Design, 9, 1139–1149, (2003)
  • Nord M., Schubert K., Cassel T., Andersson O., Riise G.: Decreased serum and bronchoalveolar lavage levels of Clara cell secretory protein (CC16) is associated with bronchiolitis obliterans syndrome and airway neutrophilia in lung transplant recipients. Transplantation, 73, 1264–1269, (2002)
  • Petrek M., Hermans C., Kolek V., Fialova J., Bernard A.: Clara cell protein (CC16) in serum and bronchoalveolar lavage fluid of subjects exposed to asbestos. Biomarkers, 7(1), 58–67, (2002)
  • Hermans C., Petrek M., Kolek V., Weynand B., Pieters T., Lambert M., Bernard A.: Serum Clara cell protein (CC16), a marker of the integrity of the air-blood barrier in sarcoidosis. Eur Respir J, 18(3), 507–514 (2001)
  • Bernard A., Roels H., Lauwerys R., Witters R., Gielens C., Soumillion A. et al.: Human urinary protein 1: Evidence for identity with the Clara cell protein and occurrence in respiratory tract and urogenital secretions. Clin Chim Acta, 207, 239–249, (1992)
  • Bernard A., Lauwerys R., Noel A., Vandeleene B., Lambert A.: Urine protein 1: a sexdependent marker of tubular or glomerular dysfunction. Clin Chem, 35, 2141–2142, (1989)
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