United States set
Menu Shopping cart $0 Search
Manufactured by BioVendor

Mouse/Rat Corticosterone ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA
  • Species:Mouse, Rat
Cat. No. Size Price

RTC002R 96 wells (1 kit) $570,15
PubMed Product Details
Technical Data


Competitive ELISA


The BioVendor Mouse/Rat Corticosterone ELISA is a competitive immunoassay for the measurement of corticosterone in rat and mouse serum or plasma. For research use only. Not for use in diagnostic procedures.


Serum, Plasma

Sample Requirements

10 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

15 – 2250 ng/ml

Limit of Detection

6.1 ng/ml



  • RUO
  • calibration range 15-2250 ng/ml
  • limit of detection 6.1 ng/ml

Research topic

Steroid hormones, Animal studies


Corticosterone is secreted by the adrenal cortex under control of the pituitary hormone ACTH via a negative feedback mechanism. It is the most abundant circulating steroid in rats, since rodents are not able to synthesize Cortisol, the major glucocorticoid in human, as a result of lacking the enzyme C17-Hydroxylase. Corticosterone has a wide range of activities in rodents. It regulate s carbohydrate, protein and fat metabolism. It has also an influence on the hemopoietic system and reduces the total number of lymphocytes and eosinophils, but to a lesser extent than cortisol. In contrast to cortisol, corticosterone has only minimal anti-inflammatory activity. Corticosterone level in nocturnal animals like rats exhibit a distinct circadian variation with peak values in the latter portion of the day, followed by a nadir in the morning (1) and is believed to play an important role in sleep-wake cyclus (2). This is in contrast to diurnal mammals, where peak concentrations of glucocorticoids are found in the morning. Enhanced corticosterone release by female compared to male rats under basal and stress conditions has been observed (6). Determination of corticosterone in rats is of interest to facilities conducting neurophysiological research, to academic institutions and to pharmaceutical companies with drug research departments. Drugs that influence the endocrine system can increase or reduce corticosteroid production in the adrenal cortex. Rat serum corticosterone is therefore an ideal indicator of the side effects of a potential therapeutic agent. The same constellations of effects seen in rats are generally seen in human. Plasma corticosterone in rats is often used in connection with ACTH measurement as a stress indicator (3,4). The effects of chronic stress on the function of the hypothalamic-pituitary-adrenocortical system are age-dependent. Recent studies suggest that aging increases basal but not stress induced levels of corticosterone in the brain (5).

Product References (1)


  • Zoubovsky SP, Hoseus S, Tumukuntala S, Schulkin JO, Williams MT, Vorhees CV, Muglia LJ. Chronic psychosocial stress during pregnancy affects maternal behavior and neuroendocrine function and modulates hypothalamic CRH and nuclear steroid receptor expression. Transl Psychiatry. 2020 Jan 16;10(1):6. doi: 10.1038/s41398-020-0704-2. PubMed PMID: 32066677. PubMed CentralPMCID: PMC7026416. See more on PubMed
Summary References (4)

References to Corticosterone

  • Hupe JM, James AC, Payne BR, Lomber SG, Girard P, Bullier J. Cortical feedback improves discrimination between figure and background by V1, V2 and V3 neurons. Nature. 1998 Aug 20;394 (6695):784-7
  • Kitaysky AS, Kitaiskaia EV, Wingfield JC, Piatt JF. Dietary restriction causes chronic elevation of corticosterone and enhances stress response in red-legged kittiwake chicks. J Comp Physiol B. 2001 Nov;171 (8):701-9
  • Thellin O, Noel G, Khurana S, Ogle CK, Horseman ND. Stress hormone secretion and gut signal transducer (STAT) proteins after burn injury in rats. Shock. 2001 Nov;16 (5):393-7
  • Vazquez-Palacios G, Retana-Marquez S, Bonilla-Jaime H, Velazquez-Moctezuma J. Further definition of the effect of corticosterone on the sleep-wake pattern in the male rat. Pharmacol Biochem Behav. 2001 Oct-Nov;70 (2-3):305-10
Related Products Docs