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ENPP1 Human (HEK), Rabbit Polyclonal Antibody

  • Regulatory status:RUO
  • Type:Polyclonal Antibody
  • Other names:Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, E-NPP 1, Membrane component chromosome 6 surface marker 1, Phosphodiesterase I/nucleotide pyrophosphatase 1, NPP-1, Plasma-cell membrane glycoprotein, PC-1
  • Species:Human
Cat. No. Size Price
1 pc / 2 - 5 pcs / 6+ pcs

RD181124100 0.1 mg $300 / $266 / On request
PubMed Product Details
Technical Data


Polyclonal Antibody



Antibodies Applications

Source of Antigen





The antibody was raised in rabbits by immunization with the recombinant Human ENPP1 Protein.

Amino Acid Sequence

Total 840 AA, UniProt P22413 (Lys98-Asp925). MW: 96.5 kDa (calculated), migrates at ~ 110 kDa on SDS PAGE; N-terminal linker (2 extra AA), C-terminal linker (4 extra AA) and C-terminal His-tag (6 extra AA).


Species Reactivity

Human. Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized recombinant Human ENPP1 Protein.

Antibody Content

0.1 mg (determined by BCA method, BSA was used as a standard)


The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. 


Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


The lyophilized antibody remains stable and fully active until the expiry date when stored at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody SDS PAGE – to determine purity of the antibody BCA - to determine quantity of the antibody


This product is for research use only.


Research topic

Bone and cartilage metabolism, Diabetology - Other Relevant Products, Energy metabolism and body weight regulation

Summary References (3)

References to ENPP1

  • Cimpean A, Stefan C, Gijsbers R, Stalmans W, Bollen M. Substrate-specifying determinants of the nucleotide pyrophosphatases/phosphodiesterases NPP1 and NPP2 [published correction appears in Biochem J. 2017 Dec 14;474(24):4273-4274]. Biochem J. 2004;381(Pt 1):71-77. doi:10.1042/BJ20040465
  • Goldfine ID et al. (2010): Endocrine Rev. 29: 62-75.
  • Hessle L, Johnson KA, Anderson HC, Narisawa S, Sali A, Goding JW, Terkeltaub R, Millan JL. Tissue-nonspecific alkaline phosphatase and plasma cell membrane glycoprotein-1 are central antagonistic regulators of bone mineralization. Proc Natl Acad Sci U S A. 2002 Jul 9;99 (14):9445-9
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