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HIV-1 Protease E.coli

  • Regulatory status:RUO
  • Type:Active protein
  • Source:E. coli
  • Species:Human Immunodeficiency Virus 1
Cat. No. Size Price

RH1P0001 0.1 mg
PubMed Product Details
Technical Data


Active protein


Total 99 AA. MW: 10.8 kDa (monomer), protein active as dimer

Amino Acid Sequence



E. coli


Purity as determined by densitometric image analysis: >90%


14% SDS-PAGE separation of Human HIV-1 Protease
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 2.5 μg/lane


20 mM sodium acetate, 200 mM NaCl, 10% glycerol, 0.05% 2-mercaptoethanol, 1 mM EDTA, pH 5.0


Defrost at ambient temperature.


Kinetic studies, Inhibitor screening, Crystallography


On ice. Upon receipt, store the product at the temperature recommended below.


Store protein at -80°C. Protein remains stable until the expiry date when stored at -80°C. Avoid repeated freezing/thawing cycles.

Quality Control Test

SDS PAGE to determine purity of the protein.
Active site titration by tightly binding inhibitor.


Km = 15.1 μM Kcat = 30 s-1 Kcat /Km = 1981 mM-1 s-1 with peptide substrate KARVF(NO2 )VRKA (F(NO2 ) … p-nitrophenylalanine)


Research topic



Retroviral protease from HIV-1 virus is an enzyme important in the life cycle of the virus. It is expressed in the infected cells as a part of Gag-Pol polyprotein from which it is autocatalytycaly released after formation of immature viral particle. The enzyme subsequently cleaves the other parts of viral polyproteins causing the maturation of the virus. In HIV-infected patients the enzyme is a subject of intensive mutagenesis and mutants resistant to applied medcines are produced as a consequence of the seletion pressure. HIV-1 protease is active as a homodimer.

Product References (5)


  • Zhang Y, Chen X, Roozbahani GM, Guan X. Graphene oxide-based biosensing platform for rapid and sensitive detection of HIV-1 protease. Anal Bioanal Chem. 2018 Sep;410(24):6177-6185. doi: 10.1007/s00216-018-1224-2. Epub 2018 Jul 2. PubMed PMID: 29968105. PubMed CentralPMCID: PMC6159923. See more on PubMed
  • Parra A, Martin-Fonseca S, Rivas F, Reyes-Zurita FJ, Medina-O'Donnell M, Martinez A, Garcia-Granados A, Lupiañez JA, Albericio F. Semi-synthesis of acylated triterpenes from olive-oil industry wastes for the development of anticancer and anti-HIV agents. Eur J Med Chem. 2014 Mar 3;74:278-301. doi: 10.1016/j.ejmech.2013.12.049. Epub 2014 Jan 8. PubMed PMID: 24480359. See more on PubMed
  • Sasková KG, Kozísek M, Rezácová P, Brynda J, Yashina T, Kagan RM, Konvalinka J. Molecular characterization of clinical isolates of human immunodeficiency virus resistant to the protease inhibitor darunavir. J Virol. 2009 Sep;83(17):8810-8. doi: 10.1128/JVI.00451-09. Epub 2009 Jun 17. PubMed PMID: 19535439. PubMed CentralPMCID: PMC2738195. See more on PubMed
  • Ingr M, Uhlíková T, Strísovský K, Majerová E, Konvalinka J. Kinetics of the dimerization of retroviral proteases: the "fireman's grip" and dimerization. Protein Sci. 2003 Oct;12(10):2173-82. doi: 10.1110/ps.03171903. PubMed PMID: 14500875. PubMed CentralPMCID: PMC2366921. See more on PubMed
  • Lindsten K, Uhlíková T, Konvalinka J, Masucci MG, Dantuma NP. Cell-based fluorescence assay for human immunodeficiency virus type 1 protease activity. Antimicrob Agents Chemother. 2001 Sep;45(9):2616-22. doi: 10.1128/AAC.45.9.2616-2622.2001. PubMed PMID: 11502538. PubMed CentralPMCID: PMC90701. See more on PubMed
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