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Manufactured by BioVendor

hsa-miR-195-5p Two-Tailed PRIMERs

  • Regulatory status:RUO
  • Type:Two-Tailed RT/ PCR Primers
  • Species:Human
Cat. No. Size Price

RDTT0000461PRI 50 rxn/ 150 rxn
PubMed Product Details
Technical Data

Don't forget

For successful completion of the assay, we strongly recommend purchasing of our Two-Tailed cDNA Synthesis System and Two-Tailed qPCR Master Mix. The combination of these three products will provide you with the highest quality results.


Two-Tailed RT/ PCR Primers


Frozen. Upon receipt, store the product at the temperature recommended below.


Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).


Below, links to independent papers developing and using Two-Tailed PCR can be found

  • Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
  • Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
  • Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
  • Click here for more papers citing Two-tailed PCR


  • For research use only.
  • For measurements specific miRNA in biological fluids.
  • Components of the kit are provided ready to use.

Research topic

Cardiovascular disease, Oncology


MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in the pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

High expressed miR-195-5p could promote cardiac hypertrophy, whereas the suppression of miR-195-5p prevented hypertrophy of H9c2 cardiomyocytes under angiotensin II treatment. It was observed that miR-195-5p promotes cardiac hypertrophy via targeting MFN2 and FBXW7. miR-195-5p was significantly upregulated in the blood of Deep vein thrombosis patients. miR-195-5p downregulation promoted cell viability and inhibited the apoptosis of human umbilical vein endothelial cells.

The expression of miR-195-5p was decreased in non-small cell lung cancer (NSCLC). Downregulation of miR-195-5p was significantly associated with TNM stage, tumor size and lymph node metastasis. On the other hand, the expression of miR-195-5p was higher in human normal lung cell lines than in NSCLC cells. Overexpression of miR-195-5p increased cell apoptotic rate of A549 cell lines, with the expression of pro-apoptotic protein Bax up-regulated and that of the anti-apoptotic protein Bcl-2 down-regulated. Therefore miR-195-5p could be used as a potential prognostic predictor and tumor suppressor in NSCLC. MiR-195-5p/-218-5p displayed a negative correlation with baculoviral IAP repeat containing 5 expression, and acted as independent prognostic factors of poor prognosis in patients with gastric cancer. miR-195-5p plays an important anticancer role in human colorectal cancer (CRC) progression as it was significantly downregulated in CRC tissues. Patients with a low level of miR-195-5p had significantly shortened overall survival. Altered miR-195-5p in colon cancer cells led to distinct changes of proliferation, migration, invasion, and epithelial-mesenchymal transition. It was observed that the gene GDPD5 is an effector of chemoresistance and metastasis in CRC. miR-195-5p is also a potent suppressor of GDPD5 and that, as such, it significantly increases chemosensitivity and apoptosis in chemoresistant CRC cells. hTERT gene plays an important role in melanoma. hTERT expression level was inversely correlated with miR-497-5p, miR-195-5p and miR-455-3p. Overexpression of miR-497-5p, miR-195-5p and miR-455-3p inhibited A375 cell proliferation, migration and invasion, arrested the cell cycle, induced cell apoptosis and decreased hTERT expression. miR-497-5p, miR-195-5p, miR-455-3p were significantly downregulated, while hTERT was upregulated in melanoma tissues. MiR-497-5p, miR-195-5p and miR-455-3p act as tumor suppressors by targeting hTERT in melanoma A375 cells.

Summary References (8)

References to miR-195-5p

  • Feng C, Zhang L, Sun Y, Li X, Zhan L, Lou Y, Wang Y, Liu L, Zhang Y. GDPD5, a target of miR-195-5p, is associated with metastasis and chemoresistance in colorectal cancer. Biomed Pharmacother. 2018 May;101:945-952. doi: 10.1016/j.biopha.2018.03.028. Epub 2018 Mar 22. PMID: 29635904. See more on PubMed
  • Chai L, Kang XJ, Sun ZZ, Zeng MF, Yu SR, Ding Y, Liang JQ, Li TT, Zhao J. MiR-497-5p, miR-195-5p and miR-455-3p function as tumor suppressors by targeting hTERT in melanoma A375 cells. Cancer Manag Res. 2018 May 3;10:989-1003. doi: 10.2147/CMAR.S163335. PMID: 29760567; PMCID: PMC5937487. See more on PubMed
  • Jin J, Wang C, Ouyang Y, Zhang D. Elevated miR-195-5p expression in deep vein thrombosis and mechanism of action in the regulation of vascular endothelial cell physiology. Exp Ther Med. 2019 Dec;18(6):4617-4624. doi: 10.3892/etm.2019.8166. Epub 2019 Nov 4. PMID: 31807149; PMCID: PMC6878892. See more on PubMed
  • Lin X, Wang S, Sun M, Zhang C, Wei C, Yang C, Dou R, Liu Q, Xiong B. miR-195-5p/NOTCH2-mediated EMT modulates IL-4 secretion in colorectal cancer to affect M2-like TAM polarization. J Hematol Oncol. 2019 Feb 26;12(1):20. doi: 10.1186/s13045-019-0708-7. Erratum in: J Hematol Oncol. 2019 Nov 22;12(1):122. PMID: 30808369; PMCID: PMC6390326. See more on PubMed
  • Luo J, Pan J, Jin Y, Li M, Chen M. MiR-195-5p Inhibits Proliferation and Induces Apoptosis of Non-Small Cell Lung Cancer Cells by Targeting CEP55. Onco Targets Ther. 2019 Dec 24;12:11465-11474. doi: 10.2147/OTT.S226921. PMID: 31920335; PMCID: PMC6935316. See more on PubMed
  • Wang L, Qin D, Shi H, Zhang Y, Li H, Han Q. MiR-195-5p Promotes Cardiomyocyte Hypertrophy by Targeting MFN2 and FBXW7. Biomed Res Int. 2019 Jun 25;2019:1580982. doi: 10.1155/2019/1580982. PMID: 31341888; PMCID: PMC6614993. See more on PubMed
  • Zheng J, Xu T, Chen F, Zhang Y. MiRNA-195-5p Functions as a Tumor Suppressor and a Predictive of Poor Prognosis in Non-small Cell Lung Cancer by Directly Targeting CIAPIN1. Pathol Oncol Res. 2019 Jul;25(3):1181-1190. doi: 10.1007/s12253-018-0552-z. Epub 2019 Jan 12. Erratum in: Pathol Oncol Res. 2020 Jul;26(3):2015. PMID: 30637589; PMCID: PMC6614139. See more on PubMed
  • Zou J, Liao X, Zhang J, Wang L. Dysregulation of miR-195-5p/-218-5p/BIRC5 axis predicts a poor prognosis in patients with gastric cancer. J Biol Regul Homeost Agents. 2019 Sep-Oct;33(5):1377-1385. doi: 10.23812/19-146-A. PMID: 31663299. See more on PubMed
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