miREIA – miRNA enzyme immunoassay
Novel method for miRNA quantification based on immunoassay. The novel method involves hybridization of miRNA isolated from patient sample to complementary biotinylated DNA oligonucleotide probe followed by monoclonal antibody detection of perfectly matched DNA/miRNA hybrids in several different arrangements and possible methods of visualization:
- Colorimetric – miReia – miRNA enzyme immunoassay
- Chemiluminescent – miRacle – miRNA antibody/capture luminometry
Click to see flyer about miRNA quantification
miREIA (miRNA Enzyme Immunoassay) – a novel, immunoassay-based method of miRNA quantification
miRNA isolated from a patient sample is hybridized to complementary biotinylated DNA oligonucleotide probe.
The DNA/RNA hybrids are then processed in the manner of ELISA in a microtiter plate coated with a monoclonal antibody specific to perfectly matched DNA/miRNA hybrids.
After washing, the solid phase is incubated with streptavidin-HRP conjugate and the resulting complexes are visualized (after another washing step) by a chromogenic substrate.
The miREIA exhibits superior analytical specificity, limit of detection as low as 0.1 attomol/μl miRNA, excellent analytical characteristics and strong correlation with the qRT-PCR method (Pearson correlation coefficient >0.9).
miREIA can be run on common immunoassay analyzers, is compatible with standard clinical workflow, does not require amplification steps and results are obtained in less than three hours including miRNA profiling.
The assay arrangement:
- Classical ELISA format
- Array format
- Luminex beads format