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miRNA Quantification Method

miREIA – miRNA enzyme immunoassay

Novel method for miRNA quantification based on immunoassay. The novel method involves hybridization of miRNA isolated from patient sample to complementary biotinylated DNA oligonucleotide probe followed by monoclonal antibody detection of perfectly matched DNA/miRNA hybrids in several different arrangements and possible methods of visualization:

  • Colorimetric – miReia – miRNA enzyme immunoassay
  • Chemiluminescent – miRacle – miRNA antibody/capture luminometry


Click to see flyer about miRNA quantification

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miREIA (miRNA Enzyme Immunoassay) – a novel, immunoassay-based method of miRNA quantification

miRNA isolated from a patient sample is hybridized to complementary biotinylated DNA oligonucleotide probe.

The DNA/RNA hybrids are then processed in the manner of ELISA in a microtiter plate coated with a monoclonal antibody specific to perfectly matched DNA/miRNA hybrids.

After washing, the solid phase is incubated with streptavidin-HRP conjugate and the resulting complexes are visualized (after another washing step) by a chromogenic substrate.

The miREIA exhibits superior analytical specificity, limit of detection as low as 0.1 attomol/μl miRNA, excellent analytical characteristics and strong correlation with the qRT-PCR method (Pearson correlation coefficient >0.9).

miREIA can be run on common immunoassay analyzers, is compatible with standard clinical workflow, does not require amplification steps and results are obtained in less than three hours including miRNA profiling.


The assay arrangement:

  • Classical ELISA format
  • Array format
  • Luminex beads format

 

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Product Size Price
hsa-miR-100-5p miREIA

Type: miREIA – miRNA enzyme immunoassay

New RDM0039H 96 wells (1 kit)
 
Product Size Price
hsa-miR-122-5p miREIA

Type: miREIA – miRNA enzyme immunoassay

New RDM0045H 96 wells (1 kit)
 
Product Size Price
hsa-miR-126-3p miREIA

Type: miREIA – miRNA enzyme immunoassay

RDM0018H 96 wells (1 kit)
 
Product Size Price
hsa-miR-129-5p miREIA

Type: miREIA – miRNA enzyme immunoassay

RDM0031H 96 wells (1 kit)
 
Product Size Price
hsa-miR-132-3p miREIA

Type: miREIA – miRNA enzyme immunoassay

New RDM0052H 96 wells (1 kit)
 
Product Size Price
hsa-miR-137-3p miREIA

Type: miREIA – miRNA enzyme immunoassay

New RDM0037H 96 wells (1 kit)
 
Product Size Price
hsa-miR-1-3p miREIA

Type: miREIA – miRNA enzyme immunoassay

RDM0019H 96 wells (1 kit)
 
Product Size Price
hsa-miR-142-5p miREIA

Type: miREIA – miRNA enzyme immunoassay

RDM0013H 96 wells (1 kit)
 
Product Size Price
hsa-miR-145-5p miREIA

Type: miREIA – miRNA enzyme immunoassay

RDM0003H 96 wells (1 kit)
 
Product Size Price
hsa-miR-146a-5p miREIA

Type: miREIA – miRNA enzyme immunoassay

RDM0024H 96 wells (1 kit)
 
Product Size Price
hsa-miR-150-5p miREIA

Type: miREIA – miRNA enzyme immunoassay

RDM0004H 96 wells (1 kit)
 
Product Size Price
hsa-miR-155-5p miREIA

Type: miREIA – miRNA enzyme immunoassay

RDM0017H 96 wells (1 kit)
 
Product Size Price
hsa-miR-15a-5p miREIA

Type: miREIA – miRNA enzyme immunoassay

RDM0007H 96 wells (1 kit)
 
Product Size Price
hsa-miR-16-5p miREIA

Type: miREIA – miRNA enzyme immunoassay

RDM0008H 96 wells (1 kit)
 
Product Size Price
hsa-miR-191-5p miREIA

Type: miREIA – miRNA enzyme immunoassay

RDM0005H 96 wells (1 kit)
 

microRNA IN A NEW LIGHT!

microRNA IN A NEW LIGHT!

MicroRNAs (miRNAs) are new and promising potential biomarkers for diagnosis and prognosis of many diseases, which opens up a new field of goals and challenges for the BioVendor R&D team. Development of miRNA diagnostic assays is just the first step; our mission is to provide validated kits to measure individual miRNAs or diagnostically relevant miRNA “groups” supported with tools for standardized miRNA isolation, sample collection, and pretreatment.

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MicroRNAs (miRNAs) are small, non-coding RNA molecules typically containing ~22 nucleotides found in human, animals, plants and some viruses which are playing an important regulatory role in the gene translation via silencing or degradation of target mRNAs. During the RNA silencing, miRNA functions as a complementary guide which binds with its target mRNAs, followed by action of AGO proteins family which works as effectors by recruiting factors that induce translational repression, mRNA deadenylation and mRNA decay. (Minju Ha, Nature Reviews, 2014)

MicroRNAs (miRNAs) are involved in virtually all physiologic processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation. The biogenesis and function of miRNAs are tightly regulated, and their dysregulation is often associated with pathology of many diseases such as cancer, heart disease or neurological disorders. Recently, the miRNA has been proposed as powerful biomarker for prediction of the diseases, treatment response or prediction of progression.

 

ID3EAL Biofluid miRNA Panel

ID3EAL Biofluid miRNA Panel

MiRXES ID3EAL Biofluid miRNA knowledge panel was compared to equivalent products from two leading vendors using a common pool of human serum RNA. For each product, RNA extracted from 200 μL of pooled human serum was used at the start of respective workflows. Detection cut-offs were determined based on the respective manufacturers’ instructions (CT ≤ 33 for ID3EAL Biofluid Panel). Of the 3 biofluid miRNA products, the ID3EAL Biofluid Panel detected the highest number of microRNA.

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Separately, the ID3EAL Biofluid Panel was used to illustrate distinct miRNA profiles in normal and gastric cancer patient serum.
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Format of Panel

 

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Biofluid miRNA knowledge panel

 

CAT. NO. Name DESCRIPTION SAMPLES
1105152 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
6 x 96-well PCR plates
6
1105153 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
12
1105154 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
24
1105252 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (6) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
6
1105253 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (12) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
12
1105254 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (24) 4 x 24 μl of ID3EAL miRNA RT Primers 96-plex
48 x 96-well PCR plates
24

 

Choose your thermal cycler

  • Roche
  • Abi Fast
  • Abi Standard
  • Bio-Rad

Biofluid miRNA Panel Analysis Template