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Manufactured by BioVendor

Myosin (Cardiac) Light Chain Human, Rabbit Polyclonal Antibody

  • Regulatory status:RUO
  • Type:Polyclonal Antibody
  • Other names:MLC-1
  • Species:Human
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Cat. No. Size Price


RD181057050 0.05 mg
PubMed Product Details
Technical Data

Type

Polyclonal Antibody

Applications

Western blotting

Source of Antigen

E. coli

Hosts

Rabbit

Preparation

The antibody was raised in rabbits by immunization with the recombinant Human Myosin (Cardiac) Light Chain fragment. The amino acid sequence of the recombinant Human Myosin (Cardiac) Light Chain fragment is 100% homologous to the amino acid sequence of the human serum Myosin (Cardiac) Light Chain fragment.

Species Reactivity

Human. Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized recombinant Human Myosin (Cardiac) Light Chain fragment.

Antibody Content

0.05 mg (determined by BCA method, BSA was used as a standard)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.05 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody SDS PAGE – to determine purity of the antibody

Note

This product is for research use only.

Summary

Research topic

Cardiovascular disease

Summary

Human ventricular myosin light chain (MLC-I, Essential Myosin Light Chain Isoform) is a 21 kDa cardiac contractile protein, which is one of component of myosin structure. The protein MLC-I is present in the contractile apparatus and not in cytosol. In severe congestive heart failure (CHF), myofibrils may degenerate and, subsequently, the myofibrillar components may be released into the circulation. The increased blood levels of MLC-I could indicate morphological changes in CHF, myocardial infarction, structural abnormalities in viable myocytes also observed in non-ischaemic CHF, myocardial necrosis.

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