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SARS-CoV Nucleocapsid Protein E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:Severe acute respiratory syndrome coronavirus nucleoprotein, SARS-CoV N protein, SARS-CoV NP, SARS nucleocapsid phosphoprotein, SARS-NP
  • Species:SARS
Cat. No. Size Price
1 - 4 pcs / 5 - 9 pcs / 10+ pcs

RD972052100 0.1 mg $300 / $266 / On request
PubMed Product Details
Technical Data


Recombinant protein


Total 437 AA. MW: 47.8 kDa (calculated). UniProtKB acc.no. P59595. N-Terminal His-tag 16 AA.

Amino Acid Sequence



E. coli


˃ 90 % by SDS-PAGE


12% SDS-PAGE separation of SARS-CoV Nucleocapsid protein
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 5μg/lane
3. non-reduced and non-heated sample, 5μg/lane


< 1.0 EU/µg


Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05 M Acetate buffer pH= 4.0


Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited.


Western blotting


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the lyophilized protein at -80 °C. Lyophilized protein remains stable until the expiry date when stored at -80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein. Endotoxin level determination.


This product is intended for research use only.


Research topic

Immune Response, Infection and Inflammation, COVID-19


Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) caused a severe outbreak in several regions of the world in 2003. The virus is a novel coronavirus isolated from patients exhibiting atypical pneumonia and may have originated from wild animals such as civet cats in southern China. The genome of SARS-CoV is a positive-sense, single-stranded RNA whose sequence is distantly related to all known coronaviruses that infect humans and animals. Like other known coronaviruses, SARS-CoV is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. The nucleocapsid (N) protein together with the viral RNA genome form a helical core located within the viral envelope.

The severe acute respiratory syndrome coronavirus nucleocapsid protein (SARS-CoV NP), a phosphoprotein of 48 kDa, is the most abundant protein in the virus-infected cells. Its primary function is to package the ∼30 kb single stranded, 5′-capped positive strand viral genome RNA molecule into a ribonucleoprotein (RNP) complex called the capsid. Ribonucleocapsid packaging is a fundamental part of viral self-assembly and the RNP complex constitutes the essential template for replication by the RNA-dependent RNA polymerase complex. In addition, the N-protein of the SARS-CoV has been shown to modulate the host cellular machinery and may serve regulatory roles during its viral life cycle.

As the most abundantly expressed structural protein during infection, SARS-CoV NP is highly detectable in SARS patients. Therefore, this protein may serve as one of the immunodominant antigens in the early diagnosis of infection. Furthermore, researchers have suggested that antibody against the N protein could modulate cytokine responses such as IL-11; non-neutralizing antibodies against N protein were found to protect mice against lethal infection.

Product References (1)


  • Shin GC, Chung YS, Kim IS, Cho HW, Kang C. Preparation and characterization of a novel monoclonal antibody specific to severe acute respiratory syndrome-coronavirus nucleocapsid protein. Virus Res. 2006 Dec;122(1-2):109-18. doi: 10.1016/j.virusres.2006.07.004. Epub 2006 Aug 30. PubMed PMID: 16942813. PubMed CentralPMCID: PMC7114302. See more on PubMed
Summary References (18)

References to SARS-CoV

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  • Shin GC, Chung YS, Kim IS, Cho HW, Kang C. Preparation and characterization ofa novel monoclonal antibody specific to severe acute respiratorysyndrome-coronavirus nucleocapsid protein. Virus Res. 2006 Dec;122(1-2):109-18.Epub 2006 Aug 30. PubMed PMID: 16942813. See more on PubMed
  • Surjit M, Liu B, Chow VT, Lal SK. The nucleocapsid protein of severe acuterespiratory syndrome-coronavirus inhibits the activity of cyclin-cyclin-dependentkinase complex and blocks S phase progression in mammalian cells. J Biol Chem.2006 Apr 21;281(16):10669-81. Epub 2006 Jan 23. PubMed PMID: 16431923. See more on PubMed
  • Surjit M, Liu B, Kumar P, Chow VT, Lal SK. The nucleocapsid protein of theSARS coronavirus is capable of self-association through a C-terminal 209 aminoacid interaction domain. Biochem Biophys Res Commun. 2004 May 14;317(4):1030-6.PubMed PMID: 15094372. See more on PubMed
  • Tan YJ, Goh PY, Fielding BC, Shen S, Chou CF, Fu JL, Leong HN, Leo YS, Ooi EE,Ling AE, Lim SG, Hong W. Profiles of antibody responses against severe acuterespiratory syndrome coronavirus recombinant proteins and their potential use as diagnostic markers. Clin Diagn Lab Immunol. 2004 Mar;11(2):362-71. PubMed PMID:15013989; PubMed Central PMCID: PMC371215. See more on PubMed
  • Timani KA, Ye L, Ye L, Zhu Y, Wu Z, Gong Z. Cloning, sequencing, expression,and purification of SARS-associated coronavirus nucleocapsid protein forserodiagnosis of SARS. J Clin Virol. 2004 Aug;30(4):309-12. PubMed PMID:15163419. See more on PubMed
  • Woo PC, Lau SK, Wong BH, Tsoi HW, Fung AM, Chan KH, Tam VK, Peiris JS, YuenKY. Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnosis of SARS coronavirus pneumonia.J Clin Microbiol. 2004 May;42(5):2306-9. PubMed PMID: 15131220; PubMed CentralPMCID: PMC404667. See more on PubMed
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