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Manufactured by BioVendor

Stanniocalcin 1 Human HEK293

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:HEK293
  • Other names:STC1, STC
  • Species:Human
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Cat. No. Size Price


RD172095025 0.025 mg
RD172095100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 240 AA. Mw: 25.9 kDa (calculated). C-Terminal Flag-tag, 10AA (highlighted). The AA sequence is identical to Swiss-Prot- P52823.

Amino Acid Sequence

THEAEQNDSVSPRKSRVAAQNSAEVVRCLNSALQVGCGAFACLENSTCDTDGMYDICKSFLYSAAKFDTQGKAFVKESLKCIANGVTSKVFLAIRRCSTFQRMIAEVQEECYSKLNVCSIAKRNPEAITEVVQLPNHFSNRYYNRLVRSLLECDEDTVSTIRDSLMEKIGPNMASLFHILQTDHCAQTHPRADFNRRRTNEPQKLKVLLRNLRGEEDSPSHIKRTSHESAASDYKDDDDK.

His 2 to Glu 5 were confirmed by N-terminal sequencing

Source

HEK293

Purity

>95%

SDS-PAGE Gel

12% SDS-PAGE separation of Human Stanniocalcin-1
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 5μg/lane
3. non-reduced and non-heated sample, 5μg/lane

Endotoxin

< 0.1 EU/μg

Formulation

Filtered (0.4 μm) and lyophilized in 0.5 mg/mL in 20mM Tris buffer, 50 mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Applications

Western blotting, ELISA, Cell culture and/or animal studies

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Note

This product is intended for research use only.

Summary

Research topic

Bone and cartilage metabolism, Oncology, Reproduction

Summary

Stanniocalcin 1 (STC1) is the mammalian homologue of STC, which was originally identified as a calcium/phosphate-regulating hormone in bony fishes. In contrast, STC1 may play an autocrine and paracrine role with pleiotropic effects in mammals. It is expressed in a wide variety of tissues, but unexpectedly is not detected in the circulation under normal circumstances, which is possibly caused by its attaching to soluble and tethered forms of a high-affinity binding protein. STC-1 can affect calcium homeostasis, bone and muscle mass and structure, and angiogenesis through effects on osteoblasts, osteoclasts, myoblasts/myocytes, and endothelial cells in mouse model. Differential regulation of myocardial STC1 protein expression was reported in heart failure. In addition, STC1 may regulate calcium currents in cardiomyocytes and may contribute to the alterations in calcium homeostasis of the failing heart. STC1 was foud to be a selective modulator of hepatocyte growth factor (HGF)-induced endothelial migration and morphogenesis, an inhibitor of macrophage chemotaxis and chemokinesis, suppressor of progesterone and luteinization inhibitor. Together with STC-2, it may play important roles in the processes of implantation and decidualization in the rat. In terminally differentiated adipocytes, it may function as a “survival factor”, which contributes to the maintenance of the integrity of mature adipose tissue. In context with its possible role in gestation, a Big STC, a three higher-molecular-mass variant has been described. STC1 was identified as one of hypoxia-responsive genes coupled to hypoxia-driven angiogenesis. Current research indicates that STC-1 might be a useful molecular marker to detect tumor cells in blood and bone marrow from patients with various types of malignancies.

Product References (9)

References

  • Block GJ, Ohkouchi S, Fung F, Frenkel J, Gregory C, Pochampally R, DiMattia G,Sullivan DE, Prockop DJ. Multipotent stromal cells are activated to reduceapoptosis in part by upregulation and secretion of stanniocalcin-1. Stem Cells.2009 Mar;27(3):670-681. doi: 10.1002/stem.20080742. PubMed PMID: 19267325; PubMedCentral PMCID: PMC4742302. See more on PubMed
  • Ito Y, Zemans R, Correll K, Yang IV, Ahmad A, Gao B, Mason RJ. Stanniocalcin-1is induced by hypoxia inducible factor in rat alveolar epithelial cells. Biochem Biophys Res Commun. 2014 Oct 3;452(4):1091-7. doi: 10.1016/j.bbrc.2014.09.060.Epub 2014 Sep 22. PubMed PMID: 25251473; PubMed Central PMCID: PMC4188510. See more on PubMed
  • Mohammadipoor A, Lee RH, Prockop DJ, Bartosh TJ. Stanniocalcin-1 attenuatesischemic cardiac injury and response of differentiating monocytes/macrophages to inflammatory stimuli. Transl Res. 2016 Nov;177:127-142. doi:10.1016/j.trsl.2016.06.011. Epub 2016 Jul 9. PubMed PMID: 27469269; PubMedCentral PMCID: PMC5099094. See more on PubMed
  • Roddy GW, Rosa RH Jr, Oh JY, Ylostalo JH, Bartosh TJ Jr, Choi H, Lee RH,Yasumura D, Ahern K, Nielsen G, Matthes MT, LaVail MM, Prockop DJ.Stanniocalcin-1 rescued photoreceptor degeneration in two rat models of inheritedretinal degeneration. Mol Ther. 2012 Apr;20(4):788-97. doi: 10.1038/mt.2011.308. Epub 2012 Jan 31. PubMed PMID: 22294148; PubMed Central PMCID: PMC3321604. See more on PubMed
  • Roddy GW, Viker KB, Winkler NS, Bahler CK, Holman BH, Sheikh-Hamad D, RoyChowdhury U, Stamer WD, Fautsch MP. Stanniocalcin-1 Is an Ocular HypotensiveAgent and a Downstream Effector Molecule That Is Necessary for the IntraocularPressure-Lowering Effects of Latanoprost. Invest Ophthalmol Vis Sci. 2017 May1;58(5):2715-2724. doi: 10.1167/iovs.16-21004. PubMed PMID: 28538979; PubMedCentral PMCID: PMC5444548. See more on PubMed
  • Roswall P, Bocci M, Bartoschek M, Li H, Kristiansen G, Jansson S, Lehn S,Sjölund J, Reid S, Larsson C, Eriksson P, Anderberg C, Cortez E, Saal LH,Orsmark-Pietras C, Cordero E, Haller BK, Häkkinen J, Burvenich IJG, Lim E, Orimo A, Höglund M, Rydén L, Moch H, Scott AM, Eriksson U, Pietras K.Microenvironmental control of breast cancer subtype elicited through paracrineplatelet-derived growth factor-CC signaling. Nat Med. 2018 May;24(4):463-473.doi: 10.1038/nm.4494. Epub 2018 Mar 12. PubMed PMID: 29529015; PubMed CentralPMCID: PMC5896729. See more on PubMed
  • Tang SE, Wu CP, Wu SY, Peng CK, Perng WC, Kang BH, Chu SJ, Huang KL.Stanniocalcin-1 ameliorates lipopolysaccharide-induced pulmonary oxidativestress, inflammation, and apoptosis in mice. Free Radic Biol Med. 2014Jun;71:321-31. doi: 10.1016/j.freeradbiomed.2014.03.034. Epub 2014 Mar 28. PubMedPMID: 24685991. See more on PubMed
  • Westberg JA, Jiang J, Andersson LC. Stanniocalcin 1 binds hemin through apartially conserved heme regulatory motif. Biochem Biophys Res Commun. 2011 Jun3;409(2):266-9. doi: 10.1016/j.bbrc.2011.05.002. Epub 2011 May 6. PubMed PMID:21570950. See more on PubMed
  • Zhao M, Xie W, Tsai SH, Hein TW, Rocke BA, Kuo L, Rosa RH Jr. IntravitrealStanniocalcin-1 Enhances New Blood Vessel Growth in a Rat Model of Laser-Induced Choroidal Neovascularization. Invest Ophthalmol Vis Sci. 2018 Feb1;59(2):1125-1133. doi: 10.1167/iovs.17-23083. PubMed PMID: 29490350; PubMedCentral PMCID: PMC5830987. See more on PubMed
Summary References (18)

References to Stanniocalcin 1

  • Arigami T, Uenosono Y, Ishigami S, Hagihara T, Haraguchi N, Matsushita D, Yanagita S, Nakajo A, Okumura H, Hokita S, Natsugoe S. Expression of stanniocalcin 1 as a potential biomarker of gastric cancer. Oncology. 2012;83 (3):158-64
  • Chang AC, Doherty J, Huschtscha LI, Redvers R, Restall C, Reddel RR, Anderson RL. STC1 expression is associated with tumor growth and metastasis in breast cancer. Clin Exp Metastasis. 2015 Jan;32 (1):15-27
  • Chang AC, Jeffrey KJ, Tokutake Y, Shimamoto A, Neumann AA, Dunham MA, Cha J, Sugawara M, Furuichi Y, Reddel RR. Human stanniocalcin (STC): genomic structure, chromosomal localization, and the presence of CAG trinucleotide repeats. Genomics. 1998 Feb 1;47 (3):393-8
  • dos Santos MT, Trindade DM, Goncalves Kde A, Bressan GC, Anastassopoulos F, Yunes JA, Kobarg J. Human stanniocalcin-1 interacts with nuclear and cytoplasmic proteins and acts as a SUMO E3 ligase. Mol Biosyst. 2011 Jan;7 (1):180-93
  • Du YZ, Gu XH, Li L, Gao F. The diagnostic value of circulating stanniocalcin-1 mRNA in non-small cell lung cancer. J Surg Oncol. 2011 Dec;104 (7):836-40
  • Fang Z, Tian Z, Luo K, Song H, Yi J. Clinical significance of stanniocalcin expression in tissue and serum of gastric cancer patients. Chin J Cancer Res. 2014 Oct;26 (5):602-10
  • Filvaroff EH, Guillet S, Zlot C, Bao M, Ingle G, Steinmetz H, Hoeffel J, Bunting S, Ross J, Carano RA, Powell-Braxton L, Wagner GF, Eckert R, Gerritsen ME, French DM. Stanniocalcin 1 alters muscle and bone structure and function in transgenic mice. Endocrinology. 2002 Sep;143 (9):3681-90
  • Fujiwara Y, Sugita Y, Nakamori S, Miyamoto A, Shiozaki K, Nagano H, Sakon M, Monden M. Assessment of Stanniocalcin-1 mRNA as a molecular marker for micrometastases of various human cancers. Int J Oncol. 2000 Apr;16 (4):799-804
  • Hasilo CP, McCudden CR, Gillespie JR, James KA, Hirvi ER, Zaidi D, Wagner GF. Nuclear targeting of stanniocalcin to mammary gland alveolar cells during pregnancy and lactation. Am J Physiol Endocrinol Metab. 2005 Oct;289 (4):E634-42
  • Hasselblatt M, Bohm C, Tatenhorst L, Dinh V, Newrzella D, Keyvani K, Jeibmann A, Buerger H, Rickert CH, Paulus W. Identification of novel diagnostic markers for choroid plexus tumors: a microarray-based approach. Am J Surg Pathol. 2006 Jan;30 (1):66-74
  • James K, Seitelbach M, McCudden CR, Wagner GF. Evidence for stanniocalcin binding activity in mammalian blood and glomerular filtrate. Kidney Int. 2005 Feb;67 (2):477-82
  • Jiang WQ, Chang AC, Satoh M, Furuichi Y, Tam PP, Reddel RR. The distribution of stanniocalcin 1 protein in fetal mouse tissues suggests a role in bone and muscle development. J Endocrinol. 2000 May;165 (2):457-66
  • Kanellis J, Bick R, Garcia G, Truong L, Tsao CC, Etemadmoghadam D, Poindexter B, Feng L, Johnson RJ, Sheikh-Hamad D. Stanniocalcin-1, an inhibitor of macrophage chemotaxis and chemokinesis. Am J Physiol Renal Physiol. 2004 Feb;286 (2):F356-62
  • Kim SJ, Ko JH, Yun JH, Kim JA, Kim TE, Lee HJ, Kim SH, Park KH, Oh JY. Stanniocalcin-1 protects retinal ganglion cells by inhibiting apoptosis and oxidative damage. PLoS One. 2013;8 (5):e63749
  • Lal A, Peters H, St Croix B, Haroon ZA, Dewhirst MW, Strausberg RL, Kaanders JH, van der Kogel AJ, Riggins GJ. Transcriptional response to hypoxia in human tumors. J Natl Cancer Inst. 2001 Sep 5;93 (17):1337-43
  • Lee CW, Hwang I, Park CS, Lee H, Park DW, Kang SJ, Lee SW, Kim YH, Park SW, Park SJ. Expression of stanniocalcin-1 in culprit coronary plaques of patients with acute myocardial infarction or stable angina. J Clin Pathol. 2013 Sep;66 (9):787-91
  • Liu G, Yang G, Chang B, Mercado-Uribe I, Huang M, Zheng J, Bast RC, Lin SH, Liu J. Stanniocalcin 1 and ovarian tumorigenesis. J Natl Cancer Inst. 2010 Jun 2;102 (11):812-27
  • Madsen KL, Tavernini MM, Yachimec C, Mendrick DL, Alfonso PJ, Buergin M, Olsen HS, Antonaccio MJ, Thomson AB, Fedorak RN. Stanniocalcin: a novel protein regulating calcium and phosphate transport across mammalian intestine. Am J Physi
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