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Manufactured by BioVendor

Anti-His Tag

  • Regulatory status:RUO
  • Type:Monoclonal Antibody
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Cat. No. Size Price


RET001 0.1 mg
PubMed Product Details
Technical Data

Type

Monoclonal Antibody

Applications

Western blotting, ELISA, Immunoprecipitation, Dot, IS

Hosts

Mouse

Isotype

IgG2b

Clone

HIS.H8 / EH158

Preparation

WB (with ECL): 1:1000–3000 dilution (incubate for one hour at room temperature) IS: 1:500–2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.

Purification Method

Protein A affinity chromatography from mouse ascites fluid.

Formulation

1 mg/mL in 10 mM PBS, pH 7.2, with 0.05% NaN3.

Storage/Expiration

Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1–2 weeks.

Summary

Summary

Anti-His Tag (HIS.H8 / EH158) Monoclonal Antibody.

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