Type
Monoclonal Antibody
Applications
Western blotting, ELISA, Immunoprecipitation, Dot, IS
Hosts
Mouse
Isotype
IgG2b
Clone
HIS.H8 / EH158
Preparation
WB (with ECL): 1:1000–3000 dilution (incubate for one hour at room temperature) IS: 1:500–2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Purification Method
Protein A affinity chromatography from mouse ascites fluid.
Formulation
1 mg/mL in 10 mM PBS, pH 7.2, with 0.05% NaN3.
Storage/Expiration
Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1–2 weeks.