Western blotting, ELISA, Dot, IS, Immunoprecipitation
WB (with ECL): 1:1000–3000 dilution (incubate for 1 hour at room temperature) IS: 1:500–2000 dilution In order to obtain best results in other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Protein A affinity chromatography from mouse ascites fluid.
1 mg/mL in 10mM PBS, pH 7.2, with 0.05% NaN3.
Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1–2 weeks.