Two-Tailed RT/ PCR Primers
Frozen. Upon receipt, store the product at the temperature recommended below.
Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).
Below, links to independent papers developing and using Two-Tailed PCR can be found
- Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
- Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
- Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
- Click here for more papers citing Two-tailed PCR
- For research use only.
- For measurements specific miRNA in biological fluids.
- Components of the kit are provided ready to use.
Cardiovascular disease, Diabetology - Other Relevant Products, Oncology, Pulmonary diseases
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.
MiR-423-5p is located within the first intron of nuclear speckle splicing regulatory protein 1 gene (NSRP1) on chromosome 17 and has been reported as an oncogenic factor in many cancers including glioblastoma, gastric cancer and prostate cancer. In contrast, miR-423-5p functions as a tumor suppressor and may serve as a diagnostic indicator in ovarian cancer and osteosarcoma.
MiR-423-5p was originally identified as a circulating biomarker for heart disease. Tijsen et al. demonstrated that circulating levels of miR-423-5p were increased in patients with clinical heart failure. Other studies suggest that miR-423-5p is enriched in pericardial fluid, and serum miR-423-5p may be associated with unstable angina pectoris. Furthermore, cellular miR-423-5p may discriminate stable coronary artery disease (CAD) patients from unstable CAD patients at six months post- acute myocardial infarction.
Additionally, it was demonstrated that miR-423–5p expression was markedly increased in blood plasma of pregnant women with preeclampsia compared with healthy control group.
Moreover, miR-423-5p is also involved in hepatic glucose and lipid metabolism. Hepatic miR-423-5p inhibition suppressed gluconeogenesis and improved insulin resistance, hyperglycemia, and fatty liver in obese diabetic mice.
Lastly, miR-423-5p has been reported as a reliable diagnostic biomarker for active tuberculosis.