Sandwich ELISA, HRP-labelled antibody
Tissue homogenates, Cerebrospinal fluid, Cell culture supernatant
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box). Do not freeze.
Limit of Detection
- The total assay time is 4 hours
Amyloid beta (Aβ or A beta) is a protein fragment of 39–43 amino acids that is the main constituent of amyloid plaques in the brains of Alzheimer's disease patients. Aβ is formed after sequential cleavage of the amyloid precursor protein (APP, transmembrane glycoprotein) by the β- and γ-secretases. . The major species generated are Aβ40 and Aβ42. The latter is more hydrophobic and more apt to aggregate and thus is considered to be primarily pathogenic, consistent with the phenotype of the major familial ADcausing mutations. Increases in either total Aβ levels or the relative concentration of the 42-amino acid form have been implicated in the pathogenesis of both familial and sporadic Alzheimer's disease. The 42-mers are the most amyloidogenic form of the peptide.