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Peptidase inhibitor 16 Human HEK293

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:HEK293
  • Other names:PI-16, Cysteine-rich secretory protein 9, CRISP-9, PSP94-binding protein, PSP94-BP, PI16, CRISP9, PSPBP, Peptidase inhibitor 16
  • Species:Human
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Cat. No. Size Price


RD172165100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 426 AA. MW 45.7 kDa (calculated), C-Terminal Flag-tag (11 extra AA). UniProt entry Q6UXB8.

Amino Acid Sequence

LTDEEKRLMVELHNLYRAQVSPPASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSAATCSPGQMCGHYTQVVWAKTERIGCGSHFCEKLQGVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYHCKNSLCEPIGSPEDAQDLPYLVTEAPSFRATEASDSRKMGTPSSLATGIPAFLVTEVSGSLATKALPAVETQAPTSLATKDPPSMATEAPPCVTTEVPSILAAHSLPSLDEEPVTFPKSTHVPIPKSADKVTDKTKVPSRSPENSLDPKMSLTGARELLPHAQEEAEAEAELPPSSEVLASVFPAQDKPGELQATLDHTGHTSSKSLPNFPNTSATANATGGRALALQSSLPGAEGPDKPSVVSGLNSGPGAAADYKDDDDK

Source

HEK293

Purity

Purity as determined by densitometric image analysis > 95%

SDS-PAGE Gel

12% SDS-PAGE separation of Human Peptidase inhibitor 16
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and boiled sample, 5 μg / lane
3. non-reduced and non-boiled sample, 5 μg / lane

Endotoxin

< 0.1 EU/μg

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Applications

Western blotting, ELISA, Cell culture and/or animal studies

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Summary

Research topic

Oncology

Summary

PSP94BP (PSPBP, Peptidase inhibitor 16, CRISP-9, PI-16) is a cell-surface-expressed type II transmembrane serine protease and was identified as one of the most highly upregulated genes in prostate cancer. PSP94BP protein expression was highest at sites of bone metastasis and in late-stage primary tumor, suggesting a role in tumor progression. In accordance, in vivo studies demonstrated that overexpression of PSP94BP led to tumor progression and metastasis. As PSP94BP expression correlates well with prostate cancer progression, it was proposed as a diagnostic marker and target for prostate cancer therapy.

Summary References (2)

References to Peptidase inhibitor 16

  • Ganesan R. An allosteric anti-PSP94BP antibody derived from a constrained phage display library. Protein engineering, design an. 2012-03;25:127-33
  • Koschubs T. Allosteric antibody inhibition of human hepsin protease. Biochem. J.. 2012-03-15;442:483-94
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