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Interleukin-6 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-6, Interferon-beta 2, B-cell stimulatory factor 2,HGF
  • Species:Human
Cat. No. Size Price

RD194015200R 96 wells (1 kit) $474
PubMed Product Details
Technical Data

Successful participation in EQA!

We are pleased to announce that we have successfully participated in the External Quality Assessment (RfB, survey of November 12, 2022), being involved in determination of IL-6.

Please find the Certificate in Docs.


Sandwich ELISA, Biotin-labelled antibody


Serum, Urine, Cerebrospinal fluid, Bronchoalveolar lavage, Amniotic fluid, Plasma, COVID-19


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1.25 – 80 pg/ml

Limit of Detection

0.65 pg/ml

Intra-assay (Within-Run)

n = 8
CV = 4.7%

Inter-assay (Run-to-Run)

n = 6
CV = 4.9%

Spiking Recovery


Dilution Linearity

Serum = 91.2%
Urine = 93.3 %
CSF = 84.5%
Amniotic fluid = 99.4%


The kits are CE-IVD certified and intended for professional use.

More about Interleukin-6 on BioVendor Scientific Blog



  • The total assay time is less than 3.5 hours
  • The kit measures IL-6 in human serum, plasma (EDTA, citrate, heparin), urine, bronchoalveolar lavage fluid (BALF), cerebrospinal fluid (CSF) and amniotic fluid
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation, Sepsis, COVID-19


Interleukin 6 (IL-6) also named B-cell stimulatory factor (BSF-2), hepatocyte-stimulating factor (HSF), hybridoma growth factor (HGF) or interferon (IFN)-β2 is a 26 kDa glycoprotein composed of 184 amino acids. IL-6 is cytokine with multiple pleiotropic effect on inflammation, immune response, hematopoiesis and oncogenesis. IL-6 is expressed in many types of cells, such as adipocytes, fibroblasts, osteoblasts, endothelial cells, neurons, lymphocytes, monocytes, neutrophils etc. and its expression is dependent on cell activation. Circulating IL-6 levels in healthy people are very low, about 1 pg/ml, but during inflammation, IL-6 levels increase rapidly. Anti-inflammatory properties of IL-6 are mainly responsible for the antibacterial and beneficial regenerative processes. Pro-inflammatory properties of IL-6 negatively affect chronic inflammation and autoimmunity. Increased levels of IL-6 have been found in patients with atherosclerotic cardiovascular disease, insulin resistant, diabetes mellitus type 2, atopic asthma, rheumatoid arthritis but also in patients with advance stage cancer, blood circulating micrometastasis etc.

Product References (9)


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Summary References (7)

References to Interleukin-6

  • Cayphas S, Van Damme J, Vink A, Simpson RJ, Billiau A, Van Snick J. Identification of an interleukin HP1-like plasmacytoma growth factor produced by L cells in response to viral infection. J Immunol. 1987 Nov 1;139 (9):2965-9
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  • Koj A. The Role of Interleukin-6 as the Hepatocyte Stimulating Factor in the Network of Inflammatory Cytokines. Annals of the New York Academy. (1989);:557: 1–8
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