Sandwich ELISA, Biotin-labelled antibody
Serum, Urine, Cerebrospinal fluid, Bronchoalveolar lavage, Amniotic fluid, Plasma
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
1.25 – 80 pg/ml
Limit of Detection
n = 8
CV = 4.7%
n = 6
CV = 4.9%
Serum = 91.2%
Urine = 93.3 %
CSF = 84.5%
Amniotic fluid = 99.4%
- The total assay time is less than 3.5 hours
- The kit measures IL-6 in human serum, plasma (EDTA, citrate, heparin), urine, bronchoalveolar lavage fluid (BALF), cerebrospinal fluid (CSF) and amniotic fluid
- Assay format is 96 wells
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation, Sepsis
Interleukin 6 (IL-6) also named B-cell stimulatory factor (BSF-2), hepatocyte-stimulating factor (HSF), hybridoma growth factor (HGF) or interferon (IFN)-β2 is a 26 kDa glycoprotein composed of 184 amino acids. IL-6 is cytokine with multiple pleiotropic effect on inflammation, immune response, hematopoiesis and oncogenesis. IL-6 is expressed in many types of cells, such as adipocytes, fibroblasts, osteoblasts, endothelial cells, neurons, lymphocytes, monocytes, neutrophils etc. and its expression is dependent on cell activation. Circulating IL-6 levels in healthy people are very low, about 1 pg/ml, but during inflammation, IL-6 levels increase rapidly. Anti-inflammatory properties of IL-6 are mainly responsible for the antibacterial and beneficial regenerative processes. Pro-inflammatory properties of IL-6 negatively affect chronic inflammation and autoimmunity. Increased levels of IL-6 have been found in patients with atherosclerotic cardiovascular disease, insulin resistant, diabetes mellitus type 2, atopic asthma, rheumatoid arthritis but also in patients with advance stage cancer, blood circulating micrometastasis etc.