Successful participation in EQA!
We are pleased to announce that we have successfully participated in the External Quality Assessment (RfB, survey of November 12, 2022), being involved in determination of IL-6.
Please find the Certificate in Docs.
Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Urine, Cerebrospinal fluid, Bronchoalveolar lavage, Amniotic fluid, Plasma
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
1.25 – 80 pg/ml
Limit of Detection
0.65 pg/ml
Intra-assay (Within-Run)
n = 8
CV = 4.7%
Inter-assay (Run-to-Run)
n = 6
CV = 4.9%
Spiking Recovery
101.3%
Dilution Linearity
Serum = 91.2%
Urine = 93.3 %
CSF = 84.5%
Amniotic fluid = 99.4%
Note
The kits are CE-IVD certified and intended for professional use.
More about Interleukin-6 on BioVendor Scientific Blog
Features
- The total assay time is less than 3.5 hours
- The kit measures IL-6 in human serum, plasma (EDTA, citrate, heparin), urine, bronchoalveolar lavage fluid (BALF), cerebrospinal fluid (CSF) and amniotic fluid
- Assay format is 96 wells
- Standard is recombinant protein based
- Components of the kit are provided ready to use, concentrated or lyophilized
Research topic
Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation, Sepsis, COVID-19
Summary
Interleukin 6 (IL-6) also named B-cell stimulatory factor (BSF-2), hepatocyte-stimulating factor (HSF), hybridoma growth factor (HGF) or interferon (IFN)-β2 is a 26 kDa glycoprotein composed of 184 amino acids. IL-6 is cytokine with multiple pleiotropic effect on inflammation, immune response, hematopoiesis and oncogenesis. IL-6 is expressed in many types of cells, such as adipocytes, fibroblasts, osteoblasts, endothelial cells, neurons, lymphocytes, monocytes, neutrophils etc. and its expression is dependent on cell activation. Circulating IL-6 levels in healthy people are very low, about 1 pg/ml, but during inflammation, IL-6 levels increase rapidly. Anti-inflammatory properties of IL-6 are mainly responsible for the antibacterial and beneficial regenerative processes. Pro-inflammatory properties of IL-6 negatively affect chronic inflammation and autoimmunity. Increased levels of IL-6 have been found in patients with atherosclerotic cardiovascular disease, insulin resistant, diabetes mellitus type 2, atopic asthma, rheumatoid arthritis but also in patients with advance stage cancer, blood circulating micrometastasis etc.
