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Interleukin-6 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:IL-6, Interferon-beta 2, B-cell stimulatory factor 2,HGF
  • Species:Human
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Cat. No. Size Price


RD194015200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Successful participation in EQA!

We are pleased to announce that we have successfully participated in the External Quality Assessment (RfB, survey of March 28, 2019), being involved in determination of IL-6.

Please find the Certificate in Docs or see article in RnD News.

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Urine, Cerebrospinal fluid, Bronchoalveolar lavage, Amniotic fluid, Plasma, COVID-19

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1.25 – 80 pg/ml

Limit of Detection

0.65 pg/ml

Intra-assay (Within-Run)

n = 8
CV = 4.7%

Inter-assay (Run-to-Run)

n = 6
CV = 4.9%

Spiking Recovery

101.3%

Dilution Linearity

Serum = 91.2%
Urine = 93.3 %
CSF = 84.5%
Amniotic fluid = 99.4%

Note

More about Interleukin-6 on BioVendor Scientific Blog

Summary

Features

  • The total assay time is less than 3.5 hours
  • The kit measures IL-6 in human serum, plasma (EDTA, citrate, heparin), urine, bronchoalveolar lavage fluid (BALF), cerebrospinal fluid (CSF) and amniotic fluid
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation, Sepsis, COVID-19

Summary

Interleukin 6 (IL-6) also named B-cell stimulatory factor (BSF-2), hepatocyte-stimulating factor (HSF), hybridoma growth factor (HGF) or interferon (IFN)-β2 is a 26 kDa glycoprotein composed of 184 amino acids. IL-6 is cytokine with multiple pleiotropic effect on inflammation, immune response, hematopoiesis and oncogenesis. IL-6 is expressed in many types of cells, such as adipocytes, fibroblasts, osteoblasts, endothelial cells, neurons, lymphocytes, monocytes, neutrophils etc. and its expression is dependent on cell activation. Circulating IL-6 levels in healthy people are very low, about 1 pg/ml, but during inflammation, IL-6 levels increase rapidly. Anti-inflammatory properties of IL-6 are mainly responsible for the antibacterial and beneficial regenerative processes. Pro-inflammatory properties of IL-6 negatively affect chronic inflammation and autoimmunity. Increased levels of IL-6 have been found in patients with atherosclerotic cardiovascular disease, insulin resistant, diabetes mellitus type 2, atopic asthma, rheumatoid arthritis but also in patients with advance stage cancer, blood circulating micrometastasis etc.

Product References (3)

References

  • Felding I, Kristoffersson U, Sjöström H, Norén O. Contribution to the 18q-syndrome. A patient with del(18) (q22.3qter). Clin Genet. 1987 Apr;31(4):206-10. PubMed PMID: 3109789. See more on PubMed
  • Prystupa A, Kiciński P, Luchowska-Kocot D, Błażewicz A, Niedziałek J, MizerskiG, Jojczuk M, Ochal A, Sak JJ, Załuska W. Association between Serum SeleniumConcentrations and Levels of Proinflammatory and ProfibroticCytokines-Interleukin-6 and Growth Differentiation Factor-15, in Patients withAlcoholic Liver Cirrhosis. Int J Environ Res Public Health. 2017 Apr 21;14(4).pii: E437. doi: 10.3390/ijerph14040437. PubMed PMID: 28430124; PubMed CentralPMCID: PMC5409638. See more on PubMed
  • Siddiqui S, Gurung RL, Liu S, Ping Seet EC, Lim SC. Genetic Polymorphisms and Cytokine Profile of Different Ethnicities inSeptic Shock Patients and theirAssociation with Mortality. Indian J Crit Care Med. 2019 Mar;23(3):135-138. doi: 10.5005/jp-journals-10071-23136. PubMed PMID: 31097890; PubMed Central PMCID:PMC6487620. See more on PubMed
Summary References (7)

References to Interleukin-6

  • Cayphas S, Van Damme J, Vink A, Simpson RJ, Billiau A, Van Snick J. Identification of an interleukin HP1-like plasmacytoma growth factor produced by L cells in response to viral infection. J Immunol. 1987 Nov 1;139 (9):2965-9
  • Hirano T, Akira S, Taga T, Kishimoto T. Biological and clinical aspects of interleukin 6. Immunol Today. 1990 Dec;11 (12):443-9
  • Hirano T, Kishimoto T. Interleukin-6.Handbook of experimental pharmacology, Peptide Growth Factors and Their Receptors. Springer-Verlag. 1990;:633-665
  • Hirano T, Taga T, Nakano N, Yasukawa K, Kashiwamura S, Shimizu K, Nakajima K, Pyun KH, Kishimoto T. Purification to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2). Proc Natl Acad Sci U S A. 1985 Aug;82 (16):5490-4
  • Koj A. The Role of Interleukin-6 as the Hepatocyte Stimulating Factor in the Network of Inflammatory Cytokines. Annals of the New York Academy. (1989);:557: 1–8
  • Nordan RP, Potter M. A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro. Science. 1986 Aug 1;233 (4763):566-9
  • Ray A, Tatter SB, Santhanam U, Helfgott DC, May LT, Sehgal PB. Regulation of expression of interleukin-6. Molecular and clinical studies. Ann N Y Acad Sci. 1989;557:353-61; discussion 361-2
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